Liu Shuang, Liu Yafang, Chen Bin, Lu Xinyan, Jiang Dandan, Geng Ling, Wang Xuan, Peng Kexin, Du Chunhong, Ren Tianguang, Yang Xing
Integrated Laboratory of Pathogenic Biology, College of Preclinical Medicine, Dali University, Dali, 671000, People's Republic of China.
School of Public Health, Dali University, Dali, 671000, China.
Parasitol Res. 2023 Nov;122(11):2609-2620. doi: 10.1007/s00436-023-07959-4. Epub 2023 Sep 9.
Trematodes can adversely impact the health and survival of wild animals. The trematode family Cyclocoelidae, which includes large digenean bird parasites, lacks molecular analysis, and reclassifications have not been supported. This study produced the first fully assembled and annotated mitochondrial genome sequence for the trematode Morishitium polonicum. The whole length of the M. polonicum (GenBank accession number: OP930879) mitogenome is 14083 bp, containing 22 transfer ribonucleic acids (tRNAs), 2 ribosomal RNAs (rRNAs, rrnL and rrnS), and a noncoding control section (D-loop) 13777 to 13854 bp in length. The 12 PCG areas have 3269 codons and a total length of 10053 bp, which makes up 71.38% of the mitochondrial genomes overall sequence. Most (10/12) of the PCGs that code for proteins begin with ATG, while the nad4L and nad1 genes have a GTG start codon. Phylogenetic analysis using the concatenated nucleotide sequences of 12 PCGs, and the ML tree analysis results showed that M. polonicum is more closely related to with Echinostomatidae and Fasciolidae, which indicates that the family Cyclocoelidae is more closely associated with Echinochasmidae. This study provides mtDNA information, and analysis of mitogenomic structure and evolution. Moreover, we aimed to understand the phylogenetic relationships of this fluke.
吸虫会对野生动物的健康和生存产生不利影响。吸虫科（Cyclocoelidae）包括大型复殖吸虫鸟类寄生虫，缺乏分子分析，且其重新分类未得到支持。本研究首次生成了吸虫波兰莫里吸虫（Morishitium polonicum）的完整组装和注释线粒体基因组序列。波兰莫里吸虫（GenBank登录号：OP930879）线粒体基因组全长14083 bp，包含22个转移核糖核酸（tRNA）、2个核糖体RNA（rRNA，rrnL和rrnS）以及一个长度为13777至13854 bp的非编码控制区（D环）。12个蛋白质编码基因（PCG）区域有3269个密码子，总长度为10053 bp，占线粒体基因组总序列的71.38%。大多数（10/12）编码蛋白质的PCG以ATG起始，而nad4L和nad1基因有GTG起始密码子。使用12个PCG的串联核苷酸序列进行系统发育分析，最大似然树（ML）分析结果表明，波兰莫里吸虫与棘口科和片形科关系更密切，这表明吸虫科与棘隙科关系更紧密。本研究提供了线粒体DNA信息，并分析了线粒体基因组结构和进化。此外，我们旨在了解这种吸虫的系统发育关系。
