National Microbiology laboratory, Winnipeg, MB, Canada.
Alberta Precision Laboratories, Public Health Laboratory, 3030 Hospital Drive N.W, Calgary, AB, T2N 4W4, Canada.
Eur J Clin Microbiol Infect Dis. 2023 Oct;42(10):1275-1280. doi: 10.1007/s10096-023-04651-4. Epub 2023 Sep 9.
To investigate the acquisition and relatedness of New Delhi Metallo-beta-lactamase among multiple separate species from one patient. Five isolates from three species (Pseudomonas aeruginosa; Pa, Acinetobacter baumannii; Ab and Proteus mirabilis; Pm) suspected of harbouring a carbapenemase were investigated by phenotype (antimicrobial susceptibilities) and whole genome sequencing. Epidemiological data was collected on this patient. Three different carbapenemase genes were detected; bla (Pa; ST773), bla (Ab, ST499) and bla identified in all isolates. NDM regions were found chromosomally integrated in all isolates. Data showed no evidence of NDM-1 transfer within this patient suggesting the enzyme was acquired in three separate events.
为了研究一位患者中多种不同种属的新德里金属β-内酰胺酶的获得和相关性。对 5 株来自 3 种不同种属（铜绿假单胞菌；Pa、鲍曼不动杆菌；Ab 和奇异变形杆菌；Pm）的分离株进行了表型（药敏试验）和全基因组测序研究，这些分离株疑似携带碳青霉烯酶。对该患者的流行病学数据进行了收集。在所有分离株中均检测到了 3 种不同的碳青霉烯酶基因；bla（Pa；ST773）、bla（Ab，ST499）和 bla。在所有分离株中均发现 NDM 区整合在染色体上。数据显示，该患者体内没有 NDM-1 转移的证据，提示该酶是在 3 次不同的事件中获得的。
