Veneto Institute of Molecular Medicine, Fondazione per la Ricerca Biomedica Avanzata, Padova, Italy.
IN-CNR, Padova, Italy.
Methods Mol Biol. 2023;2710:131-148. doi: 10.1007/978-1-0716-3425-7_11.
Cranial window implant is the preparation of choice for acute and chronic optical access to a given brain area. The cranial window provides a stable preparation, which can last for months. This window allows to follow the activity of distinct population of neurons expressing genetically encoded fluorescent reporters of activity in awake, behaving, head-fixed animals. The optical access can also be exploited for acute imaging, in anesthetized animals. Here we provide a detailed protocol for acute and chronic cranial window implantations in the olfactory bulb. We also provide the procedure to perform injections of adeno-associated viruses expressing genetically encoded fluorescent sensors in the same area.
颅窗植入是获得特定脑区急性和慢性光遗传学研究的首选方法。颅窗提供了一个稳定的实验准备，可持续数月。该窗口允许在清醒、行为、头部固定的动物中，对表达基因编码的活性荧光报告基因的特定神经元群体的活动进行跟踪。该光学方法也可用于在麻醉动物中进行急性成像。本实验提供了在嗅球进行急性和慢性颅窗植入的详细方案，同时也提供了在同一部位注射表达基因编码荧光传感器的腺相关病毒的操作流程。
