Loeffler M, Potten C S, Ditchfield A, Wichmann H E
Cell Tissue Kinet. 1986 Jul;19(4):377-89. doi: 10.1111/j.1365-2184.1986.tb00736.x.
A new cell kinetic approach is presented from which the duration of the S and G2 + M phases can be estimated. The technique involves an analysis of the spatial distribution of labelled cells in sections or sheets of epithelium (i.e. an analysis of clustered labelled cells). The technique is largely independent of the absolute number of labelled cells and hence is not influenced by factors which affect the absolute number of labelled cells. The technique is described and experimental data from dorsal murine skin are presented. The technique has also been simulated mathematically so that the phase durations and their variances could be estimated. The advantages of the technique are: it is technically simple; it provides at least two independent estimates of the phase durations; unlabelled cells need not be counted (compare with LI or PLM analysis); it is independent of variations in the absolute yield of labelled cells, and it is applicable if the LI is low and the S phase is short (where the PLM technique tends to fail).
本文提出了一种新的细胞动力学方法，可据此估算S期和G2+M期的持续时间。该技术涉及分析上皮切片或薄片中标记细胞的空间分布（即分析聚集的标记细胞）。该技术在很大程度上与标记细胞的绝对数量无关，因此不受影响标记细胞绝对数量的因素影响。本文描述了该技术，并展示了来自小鼠背部皮肤的实验数据。该技术也已通过数学模拟，以便估算各期持续时间及其方差。该技术的优点包括：技术操作简单；可提供至少两种对各期持续时间的独立估算；无需对未标记细胞进行计数（与标记指数或标记有丝分裂分析相比）；不受标记细胞绝对产率变化的影响，并且在标记指数较低且S期较短（此时标记有丝分裂技术往往失效）的情况下也适用。
