Potten C S, Wichmann H E, Dobek K, Birch J, Codd T M, Horrocks L, Pedrick M, Tickle S P
Cell Tissue Kinet. 1985 Jan;18(1):59-70. doi: 10.1111/j.1365-2184.1985.tb00632.x.
Full PLM curves have been obtained for four sites in the mouse. The first peaks have been analysed by computer and the duration of the G2 + M and S phases determined together with their standard deviations. The full curves showed a general similarity for all four sites with no clear second peak. The data are compared with the published data for mouse and human epidermis using the in vivo PLM technique. The timing and shape of the first peak can vary considerably even for one site in mice. Hence, both G2 + M and S can vary in their durations. Cells labelled at one time of day exhibit different kinetic properties to those labelled at another time of day. The duration of G2 + M is shortest in dorsum labelled at 03.00 hours (3 X 2 hr) and longest in tail (up to 7 X 5 hr). The S-phase is shortest in dorsum (6 X 3-7 X 2 hr) and longest in tail or ear (13 X 3-14 X 1 hr). There is also a very large standard deviation in tail and foot. There is little general variability when the psoriatic human data are considered, which is surprising. The general variability amongst the data from experimental mice might also be expected amongst humans which might make comparisons between the cell kinetics of normal and diseased skin difficult.
已获得小鼠四个部位的完整脉冲标记有丝分裂曲线。通过计算机分析了第一个峰值，并确定了G2 + M期和S期的持续时间及其标准差。完整曲线显示所有四个部位总体相似，没有明显的第二个峰值。使用体内脉冲标记有丝分裂技术将这些数据与已发表的小鼠和人类表皮数据进行了比较。即使是小鼠的一个部位，第一个峰值的时间和形状也可能有很大差异。因此，G2 + M期和S期的持续时间都可能不同。一天中某个时间标记的细胞与在另一个时间标记的细胞表现出不同的动力学特性。G2 + M期在凌晨3点标记的背部最短（3×2小时），在尾部最长（长达7×5小时）。S期在背部最短（6×3 - 7×2小时），在尾部或耳部最长（13×3 - 14×1小时）。尾部和足部的标准差也非常大。考虑银屑病人类数据时，总体变异性很小，这很令人惊讶。实验小鼠数据中的总体变异性在人类中可能也会出现，这可能会使正常皮肤和患病皮肤的细胞动力学比较变得困难。
