Alyahyawi Amjad R, Khan Salman, Rafi Zeeshan, Singh Parul, Moheet Kahkashan, Akasha Rihab, Ahmad Saheem
Department of Diagnostic Radiology, College of Applied Medical Science, University of Hail, Ha'il 2440, Saudi Arabia.
Centre for Nuclear and Radiation Physics, Department of Physics, University of Surrey, Guildford GU2 7XH, United Kingdom.
Saudi J Biol Sci. 2023 Oct;30(10):103782. doi: 10.1016/j.sjbs.2023.103782. Epub 2023 Aug 19.
This pioneering study aims to address the paradox of the highly regarded fruit, whose valuable peels have been considered undesired remnants from industrial fruit juice production. The study proposes the utilization of these discarded peels to synthesize ecologically safe gold nanoparticles (K-AuNPs) through a one-pot method. The objectives of this research are to synthesize K-AuNPs using an ecologically safe single-step approach, utilizing discarded fruit peels, and to assess their antibacterial and antidiabetic potential. The validation of K-AuNPs involved various techniques including UV-visible spectroscopy, TEM, DLS, and zeta-potential investigations. The antibacterial activity against and was compared to levofloxacin and aqueous peel extract (KAPE). Furthermore, the anti-diabetic efficacy was evaluated through α-amylase and α-glucosidase experiments, comparing K-AuNPs to pure KAPE and the standard inhibitor acarbose. The results confirmed the successful synthesis of K-AuNPs from KAPE, as evidenced by UV-spectral profiles (527 nm), TEM micrographs (∼21 d. nm), dynamic light scattering (65 d.nm), and zeta-potential (-12 mV). The K-AuNPs demonstrated a superior zone of inhibition and lower MIC values against and , surpassing levofloxacin and KAPE alone. Additionally, the K-AuNPs exhibited potent anti-diabetic efficacy, outperforming both pure KAPE and acarbose at a lower dosage. To sum up, the process of producing K-AuNPs utilizing peel extracts demonstrates a powerful antibacterial and antidiabetic remedy sourced from previously discarded materials. These findings signify a significant leap forward in the domain of natural product exploration, with the potential to fundamentally reshape modern healthcare approaches.
这项开创性研究旨在解决备受赞誉的水果所存在的矛盾之处，这种水果的珍贵果皮在工业果汁生产中一直被视为无用的残余物。该研究提出利用这些废弃果皮，通过一锅法合成生态安全的金纳米颗粒（K-AuNPs）。本研究的目的是采用生态安全的单步方法，利用废弃水果皮合成K-AuNPs，并评估其抗菌和抗糖尿病潜力。K-AuNPs的验证涉及多种技术，包括紫外可见光谱、透射电子显微镜、动态光散射和zeta电位研究。将其对大肠杆菌和金黄色葡萄球菌的抗菌活性与左氧氟沙星和果皮水提取物（KAPE）进行比较。此外，通过α-淀粉酶和α-葡萄糖苷酶实验评估抗糖尿病功效，将K-AuNPs与纯KAPE和标准抑制剂阿卡波糖进行比较。结果证实从KAPE成功合成了K-AuNPs，紫外光谱图（527nm）、透射电子显微镜照片（约21nm）、动态光散射（65nm）和zeta电位（-12mV）证明了这一点。K-AuNPs对大肠杆菌和金黄色葡萄球菌表现出更好的抑菌圈和更低的最低抑菌浓度值，超过了单独的左氧氟沙星和KAPE。此外，K-AuNPs表现出强大的抗糖尿病功效，在较低剂量下优于纯KAPE和阿卡波糖。总之，利用果皮提取物生产K-AuNPs的过程展示了一种源自先前废弃材料的强大抗菌和抗糖尿病疗法。这些发现标志着天然产物探索领域的重大飞跃，有可能从根本上重塑现代医疗保健方法。
