一项关于从住院患者尿液中分离出的菌种的研究，特别涉及菌种鉴定、抗真菌药敏性以及快速和传统菌种鉴定方法的比较
A Study on Urinary Isolates of Species Isolated from Hospitalized Patients with Special Reference to Speciation, Antifungal Susceptibility, and Comparison of Rapid and Conventional Methods of Speciation.
作者信息
Patel Sanjaykumar P, Nimavat Keyur, Patel Pragnesh, Kachhadia Jenish
机构信息
Department of Radiology, Banas Medical College and Research Institute, Palanpur, Gujarat, India.
Department of Forensic Medicine and Toxicology, Shantabaa Medical College and General Hospital, Amreli, Gujarat, India.
出版信息
J Pharm Bioallied Sci. 2023 Jul;15(Suppl 2):S1291-S1293. doi: 10.4103/jpbs.jpbs_114_23. Epub 2023 Jul 11.
AIM
In order to determine which species of were isolated from hospitalized patients' urine, which risk factors they were linked to, and which antifungal drugs were effective against them, the current investigation was carried out.
MATERIALS AND METHODS
One hundred inpatients were part of the study group. Urine samples were collected from each subject and spun at 3000 rpm for 10-15 minutes in individual sterile centrifuge tubes. Color, size, texture, and the presence of color diffusion into the surrounding agar were used to identify the distinct species, presumably in 48 hours.
RESULTS
A total of 86.7% of the spp. were found to be non-albicans. Major inaccuracies occurred because of differences between methodologies; thus, MBD is the best way to determine antifungal susceptibility to itraconazole and amphotericin B.
CONCLUSION
To help clinicians decide on an empirical treatment in an emergency, accurate identification of down to the species level is crucial.
目的
为了确定从住院患者尿液中分离出的是哪种[具体微生物名称未给出]，它们与哪些危险因素相关，以及哪些抗真菌药物对其有效，开展了本项调查。
材料与方法
100名住院患者作为研究组。从每个受试者收集尿液样本，并在单独的无菌离心管中以3000转/分钟的速度离心10 - 15分钟。通过颜色、大小、质地以及颜色向周围琼脂扩散的情况来鉴定不同的[具体微生物名称未给出]种类，大概在48小时内完成。
结果
总共发现86.7%的[具体微生物名称未给出]属为非白色念珠菌。由于方法之间存在差异，出现了主要的误差；因此，微量肉汤稀释法（MBD）是确定对伊曲康唑和两性霉素B的抗真菌药敏性的最佳方法。
结论
为帮助临床医生在紧急情况下做出经验性治疗决策，准确鉴定[具体微生物名称未给出]至种水平至关重要。
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