Onyia K A
Department of Physiology, School of Medicine, College of Medical Sciences, University of Benin, Nigeria.
Comp Immunol Microbiol Infect Dis. 1989;12(3):77-82. doi: 10.1016/0147-9571(89)90052-0.
In vivo chemotaxis to rat leucocytes was shown to mildly oxidised arachidonic acid in the migration into cotton pellet method. Amounts of oxidised acid, 0.25, 0.5 and 2.5 mg, mobilized net leucocytes (mostly neutrophils) into experimental pellets in 12 h. The 0.5 mg of oxidised acid showed maximum chemotactic activity and attracted maximum net leucocytes into the experimental pellets at 12 h. Doses lower or higher than this were less effective. Equivalent doses of pure arachidonic acid failed to show these chemotactic activities but evoked inflammatory reactions at the experimental pellet sites. Chemotactic activity seemed therefore to require mild oxidation of the lipid. Oxidation also seemed to stimulate spontaneous migratory activity into the control pellets.
在棉球植入法中，向大鼠白细胞的体内趋化作用表明，轻度氧化的花生四烯酸具有这种趋化作用。0.25毫克、0.5毫克和2.5毫克的氧化酸在12小时内将净白细胞（主要是中性粒细胞）募集到实验棉球中。0.5毫克的氧化酸表现出最大的趋化活性，并在12小时时将最多的净白细胞吸引到实验棉球中。低于或高于此剂量的效果较差。等量的纯花生四烯酸未显示出这些趋化活性，但在实验棉球部位引发了炎症反应。因此，趋化活性似乎需要脂质的轻度氧化。氧化似乎还刺激了向对照棉球的自发迁移活性。
