Vasta G R, Cheng T C, Marchalonis J J
Cell Immunol. 1984 Oct 15;88(2):475-88. doi: 10.1016/0008-8749(84)90179-5.
Using antisera produced against a serum lectin we have shown by employing immunocytofluorescence that hemocytes from the oyster, Crassostrea virginica, possess a lectin which is situated on the external surface of the cell membrane. The antisera block the binding of hemocyte microsomes to protease-treated vertebrate erythrocytes, thus confirming that the hemocyte membrane lectin is serologically related to the serum lectin. The major serum lectin has an apparent mass of 34,000. Flow cytometry has revealed that the distribution of the surface lectin on hemocytes represents a heterogeneous expression on a population basis, but no discrete cell subpopulations can be identified.
我们利用针对一种血清凝集素产生的抗血清，通过免疫细胞荧光法证明，来自弗吉尼亚牡蛎（Crassostrea virginica）的血细胞拥有一种位于细胞膜外表面的凝集素。这些抗血清可阻断血细胞微粒体与经蛋白酶处理的脊椎动物红细胞的结合，从而证实血细胞膜凝集素与血清凝集素在血清学上相关。主要的血清凝集素表观质量为34,000。流式细胞术显示，血细胞表面凝集素的分布在群体基础上呈现异质性表达，但无法识别出离散的细胞亚群。
