脑内移植后星形胶质细胞的种间鉴定

Interspecies identification of astrocytes after intracerebral transplantation.

作者信息

Jacque C M, Suard I M, Collins V P, Raoul M M

出版信息

Dev Neurosci. 1986;8(3):142-9. doi: 10.1159/000112249.

Abstract

Species-specificity of the Tp-GFAP 1 (glial fibrillary acidic protein) monoclonal antibodies raised against calf GFAP was established by means of immunochemical techniques. Since it was shown to combine with rabbit GFAP but not with mouse GFAP it allows the characterization of a new experimental model potentially useful in the study of the fate of implanted astrocytes after intracerebral graft of CNS fragments. Preliminary observations indicate that embryonic and newborn rabbit astrocytes are able to survive, express GFAP and migrate when implanted into newborn mouse brain.

摘要

通过免疫化学技术确定了针对小牛胶质纤维酸性蛋白（GFAP）产生的Tp - GFAP 1单克隆抗体的物种特异性。由于已证明它能与兔GFAP结合但不能与小鼠GFAP结合，所以它可用于表征一种新的实验模型，该模型可能有助于研究中枢神经系统片段脑内移植后植入的星形胶质细胞的命运。初步观察表明，胚胎期和新生兔的星形胶质细胞植入新生小鼠脑内后能够存活、表达GFAP并迁移。