绿色合成锌和镍双掺杂氧化铈纳米粒子：抗氧化活性和细胞毒性作用。
Green synthesis of zinc and nickel dual-doped cerium oxide nanoparticles: antioxidant activity and cytotoxicity effects.
机构信息
Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
出版信息
Bioprocess Biosyst Eng. 2023 Nov;46(11):1569-1578. doi: 10.1007/s00449-023-02920-2. Epub 2023 Sep 12.
Cerium oxide nanoparticles (CeO-NPs) and Zn-Ni dual-doped CeO-NPs were synthesized through a green approach by the implication of zucchini peel (Cucurbita pepo) extract as a capping and reduction agent. All the synthesized samples were studied by the results of FTIR, UV-Vis, XRD, and FESEM/EDAX/PSA analyses. The Zn-Ni dual-doped CeO-NPs contained a spherical morphology and their size was observed to increase at higher temperatures. The conducted MTT assay on the Huh-7 cell line displayed 50% of cells annihilation as a result of using undoped CeO-NPs and Zn-Ni dual-doped CeO-NPs at the inhibitory concentrations (IC) of 700 and 185.4 μg/mL, respectively. We also evaluated the enzymatic functionality of SOD and CAT of undoped CeO-NPs and dual-doped NPs and found it to be dose dependent. Moreover, Zn-Ni dual-doped CeO-NPs intensified the CAT activity without causing any changes in SOD activity in similar concentrations.
氧化铈纳米粒子（CeO-NPs）和 Zn-Ni 双掺杂氧化铈纳米粒子通过绿色方法合成，采用南瓜皮（Cucurbita pepo）提取物作为封端和还原剂。所有合成的样品均通过 FTIR、UV-Vis、XRD 和 FESEM/EDAX/PSA 分析的结果进行了研究。Zn-Ni 双掺杂氧化铈纳米粒子具有球形形态，并且随着温度的升高，其尺寸观察到增加。在 Huh-7 细胞系上进行的 MTT 测定显示，由于在抑制浓度（IC）下使用未掺杂的 CeO-NPs 和 Zn-Ni 双掺杂 CeO-NPs 分别为 700 和 185.4μg/mL，导致 50%的细胞被消灭。我们还评估了未掺杂的 CeO-NPs 和双掺杂 NPs 的 SOD 和 CAT 的酶功能，发现其具有剂量依赖性。此外，Zn-Ni 双掺杂氧化铈纳米粒子在不引起 SOD 活性变化的情况下，在相似浓度下增强了 CAT 活性。
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