Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Bioprocess Biosyst Eng. 2023 Nov;46(11):1569-1578. doi: 10.1007/s00449-023-02920-2. Epub 2023 Sep 12.
Cerium oxide nanoparticles (CeO-NPs) and Zn-Ni dual-doped CeO-NPs were synthesized through a green approach by the implication of zucchini peel (Cucurbita pepo) extract as a capping and reduction agent. All the synthesized samples were studied by the results of FTIR, UV-Vis, XRD, and FESEM/EDAX/PSA analyses. The Zn-Ni dual-doped CeO-NPs contained a spherical morphology and their size was observed to increase at higher temperatures. The conducted MTT assay on the Huh-7 cell line displayed 50% of cells annihilation as a result of using undoped CeO-NPs and Zn-Ni dual-doped CeO-NPs at the inhibitory concentrations (IC) of 700 and 185.4 μg/mL, respectively. We also evaluated the enzymatic functionality of SOD and CAT of undoped CeO-NPs and dual-doped NPs and found it to be dose dependent. Moreover, Zn-Ni dual-doped CeO-NPs intensified the CAT activity without causing any changes in SOD activity in similar concentrations.
氧化铈纳米粒子（CeO-NPs）和 Zn-Ni 双掺杂氧化铈纳米粒子通过绿色方法合成，采用南瓜皮（Cucurbita pepo）提取物作为封端和还原剂。所有合成的样品均通过 FTIR、UV-Vis、XRD 和 FESEM/EDAX/PSA 分析的结果进行了研究。Zn-Ni 双掺杂氧化铈纳米粒子具有球形形态，并且随着温度的升高，其尺寸观察到增加。在 Huh-7 细胞系上进行的 MTT 测定显示，由于在抑制浓度（IC）下使用未掺杂的 CeO-NPs 和 Zn-Ni 双掺杂 CeO-NPs 分别为 700 和 185.4μg/mL，导致 50%的细胞被消灭。我们还评估了未掺杂的 CeO-NPs 和双掺杂 NPs 的 SOD 和 CAT 的酶功能，发现其具有剂量依赖性。此外，Zn-Ni 双掺杂氧化铈纳米粒子在不引起 SOD 活性变化的情况下，在相似浓度下增强了 CAT 活性。
