Miri Abdolhossein, Sarani Mina, Khatami Mehrdad
Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences Zabol Iran.
NanoBioEletrochemistry Research Center, Bam University of Medical Sciences Bam Iran.
RSC Adv. 2020 Jan 23;10(7):3967-3977. doi: 10.1039/c9ra09076b. eCollection 2020 Jan 22.
This study was conducted to obtain Ni Ce O (where = 0, 1, 3 and 5% w/w) nanoparticles using extracts through an easy, inexpensive and non-toxic method. The biosynthesized nanoparticles have been characterized powder X-ray diffraction (PXRD), Raman spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) spectroscopy, and vibrating-sample magnetometer (VSM) analysis. The results of PXRD showed that Ni doping in the CeO process generated a higher shift at an angle of (111); also, the PXRD patterns were surveyed by the Rietveld refinement technique. Raman analysis revealed that doping nickel in CeO led to the nanoparticles reducing the intensity of the F mode. The FESEM images showed that the particle size was 5-6 nm and it had a spherical shape. The hysteresis loops of the synthesized nanoparticles were similar to that of the normal ferromagnetic materials. The cytotoxic activity of the synthesized undoped and Ni-doped CeO-NPs was determined using MTT assays against a colon cancer cell line (HT-29). The results showed that the cytotoxic effect of the synthesized nanoparticles changed after doping nickel in CeO-NPs. The increase in the Ni-doping value for CeO-NPs increased the cytotoxic activity. The sun protection factor (SPF) has been estimated through spectrophotometric measurements for determining UV protection. This showed that increasing the percentage of nickel in the doped nanoparticles increased the protection factor and a higher SPF value was obtained: 48.52.
本研究旨在通过一种简便、廉价且无毒的方法，利用提取物获得NiCeO（其中 = 0、1、3和5% w/w）纳米颗粒。通过粉末X射线衍射（PXRD）、拉曼光谱、场发射扫描电子显微镜（FESEM）、能量色散X射线（EDX）光谱和振动样品磁强计（VSM）分析对生物合成的纳米颗粒进行了表征。PXRD结果表明，在CeO过程中掺杂Ni在（111）角处产生了更高的位移；此外，还通过Rietveld精修技术对PXRD图谱进行了研究。拉曼分析表明，在CeO中掺杂镍导致纳米颗粒的F模式强度降低。FESEM图像显示，粒径为5 - 6nm，呈球形。合成纳米颗粒的磁滞回线与正常铁磁材料的磁滞回线相似。使用MTT法针对结肠癌细胞系（HT - 29）测定了合成的未掺杂和Ni掺杂的CeO - NPs的细胞毒性活性。结果表明，在CeO - NPs中掺杂镍后，合成纳米颗粒的细胞毒性作用发生了变化。CeO - NPs中Ni掺杂值的增加提高了细胞毒性活性。通过分光光度测量估计了防晒系数（SPF）以确定紫外线防护效果。结果表明，掺杂纳米颗粒中镍含量的增加提高了防护系数，获得了更高的SPF值：48.52。
