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微波辅助从砂拉越种咖啡果肉中提取生物活性化合物:统计优化及与传统方法的比较

Microwave-assisted extraction of bioactive compounds from Sarawak sp. coffee pulp: Statistical optimization and comparison with conventional methods.

作者信息

Wong Joel Ching Jue, Nillian Elexson

机构信息

Faculty of Resource Science and Technology University Malaysia Sarawak Kota Samarahan Sarawak Malaysia.

出版信息

Food Sci Nutr. 2023 Jun 11;11(9):5364-5378. doi: 10.1002/fsn3.3494. eCollection 2023 Sep.

Abstract

, commonly known as coffee, is a kind of coffee that originated in Liberia, a West African country. It is considered a less-known coffee bean variety, which accounts for less than 2% of commercially produced coffee worldwide. In this study, the influences of optimization of microwave-assisted extraction (MAE) on the total phenolic content (TPC), total flavonoid content (TFC), and total carbohydrate content (TCC) of bioactive compounds extracted from Sarawak sp. coffee pulp were studied. Response surface methodology was adopted with a face-centered central composite design to generate 34 responses by taking three microwave parameters into consideration, microwave power (watt), time of irradiation (second), and solvent-to-feed ratio as independent variables. As a result, the findings revealed that optimum extraction conditions were conducted as follows: microwave power of 700 W, time of irradiation of 180 s, and solvent-to-feed ratio of 86.644:1. While under optimal extraction conditions, MAE outperformed conventional maceration extraction in terms of extraction efficiency and had no significant difference ( < .05) with Soxhlet extraction on the extraction of TPC (12.94 ± 2.25 mg GAE/g), TFC (9.84 ± 0.38 mg QE/g), and TCC (876.50 ± 64.15 mg GE/g). Present work advances the usage of Sarawak sp. coffee for the development of functional products and aids in reducing environmental pollution by utilization of coffee pulp waste.

摘要

,通常被称为咖啡,是一种原产于西非国家利比里亚的咖啡。它被认为是一种不太知名的咖啡豆品种,在全球商业生产的咖啡中所占比例不到2%。在本研究中,研究了微波辅助萃取(MAE)优化对从砂拉越种咖啡果肉中提取的生物活性化合物的总酚含量(TPC)、总黄酮含量(TFC)和总碳水化合物含量(TCC)的影响。采用响应面法,通过面心中央复合设计,将微波功率(瓦特)、辐照时间(秒)和溶剂与进料比这三个微波参数作为自变量,生成34个响应。结果表明,最佳萃取条件如下:微波功率700W,辐照时间180s,溶剂与进料比86.644:1。在最佳萃取条件下,MAE在萃取效率方面优于传统浸渍萃取,并且在TPC(12.94±2.25mg GAE/g)、TFC(9.84±0.38mg QE/g)和TCC(876.50±64.15mg GE/g)的萃取方面与索氏萃取没有显著差异(<0.05)。目前的工作推进了砂拉越种咖啡在功能性产品开发中的应用,并有助于通过利用咖啡果肉废弃物减少环境污染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5556/10494612/c9da1b0580ad/FSN3-11-5364-g002.jpg

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