烧伤恢复大鼠的肝脏和骨骼肌磷脂代谢

Hepatic and skeletal muscle phospholipid metabolism in recovering burned rats.

作者信息

Turinsky J

出版信息

Exp Mol Pathol. 1986 Oct;45(2):185-92. doi: 10.1016/0014-4800(86)90058-4.

Abstract

Liver and soleus muscles of control animals and rats recovering from a single hindlimb scald were analyzed for diphosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline, sphingomyelin, lysophosphatidyl choline, phosphatidyl inositol, and phosphatidyl serine. Liver of 4-hr postburn rats exhibited decreased contents of diphosphatidyl glycerol (-20%), phosphatidyl ethanolamine (-11%), and phosphatidyl choline (-7%). At 3 days after the burn, only an 11% decrease in hepatic phosphatidyl inositol was observed. Soleus muscle of the unburned limb of burned rats showed at 3 days postburn an 11% decrease in sphingomyelin content but the other measured phospholipids were at control level. In contrast, soleus muscle from the contralateral burned limb exhibited increased contents of sphingomyelin (+29%), lysophosphatidyl choline (+145%), and phosphatidyl serine (+27%) compared to control uninjured animals. It also showed a 25% higher phosphatidyl inositol level than the contralateral uninjured counterpart. It is concluded that recovery from a single hindlimb scald is associated with alterations in phospholipid metabolism in the liver and in the region of the wound. The local response to thermal injury may be mediated, in part, by stimulated activity of phospholipase A2.

摘要

对对照组动物以及从单侧后肢烫伤中恢复的大鼠的肝脏和比目鱼肌进行分析，检测其中的二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、鞘磷脂、溶血磷脂酰胆碱、磷脂酰肌醇和磷脂酰丝氨酸。烧伤后4小时的大鼠肝脏中二磷脂酰甘油含量降低了20%，磷脂酰乙醇胺降低了11%，磷脂酰胆碱降低了7%。烧伤后3天，仅观察到肝脏中磷脂酰肌醇降低了11%。烧伤大鼠未烧伤肢体的比目鱼肌在烧伤后3天鞘磷脂含量降低了11%，但其他检测的磷脂处于对照水平。相比之下，与未受伤的对照动物相比，对侧烧伤肢体的比目鱼肌中鞘磷脂含量增加了29%，溶血磷脂酰胆碱增加了145%，磷脂酰丝氨酸增加了27%。其磷脂酰肌醇水平也比另一侧未受伤的对应肌肉高25%。得出的结论是，从单侧后肢烫伤中恢复与肝脏和伤口区域磷脂代谢的改变有关。对热损伤的局部反应可能部分由磷脂酶A2的活性增强介导。