基于 CRISPR 的 RNA 病毒工程。

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Sci Adv. 2023 Sep 15;9(37):eadj8277. doi: 10.1126/sciadv.adj8277. Epub 2023 Sep 13.

CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

CRISPR RNA 引导的内切酶使 DNA 的精确编辑成为可能。然而，编辑 RNA 的选择仍然有限。在这里，我们将 CRISPR 核糖核酸酶的序列特异性 RNA 切割与可编程 RNA 修复相结合，在 RNA 中进行精确的缺失和插入。这项工作建立了一种重组 RNA 技术，可立即应用于 RNA 病毒的简便工程改造。