Tau Rocío Lucía, Ferreccio Carola, Bachir Natalia, Torales Fatima, Romera Sonia Alejandra, Maidana Silvina Soledad
Institute of Virology and Technological Innovations, IVIT (INTA-CONICET), Dr Nicolas Repetto and De los Reseros, CP 1686, Hurlingham, Buenos Aires, Argentina.
Institute of Virology and Technological Innovations, IVIT (INTA-CONICET), Dr Nicolas Repetto and De los Reseros, CP 1686, Hurlingham, Buenos Aires, Argentina; Chair of immunology, University of Salvador (USAL), Champagnat 1599, CP 1630, Pilar, Buenos Aires, Argentina.
J Equine Vet Sci. 2023 Nov;130:104916. doi: 10.1016/j.jevs.2023.104916. Epub 2023 Sep 11.
High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. Ten out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes.
基因组的高通量测序扩展了我们对甲型疱疹病毒亚科的认识，这是一类广泛存在的DNA病毒亚科，它们通过重组来增加遗传多样性。人们已经认识到，对养马业有经济影响的两种甲型疱疹病毒——马疱疹病毒1型（EHV-1）和马疱疹病毒4型（EHV-4）——能够发生重组。这项研究旨在利用GenBank中提供的EHV-1、EHV-3、EHV-4、EHV-6、EHV-8和EHV-9的基因组，分析所有马属甲型疱疹病毒物种之间的种间重组。通过RDP4和Simplot软件，在EHV-1与EHV-4、EHV-1与EHV-9、EHV-8与EHV-1以及EHV-8与EHV-9之间鉴定出了14个重组事件。14个事件中有10个涉及ORF64，这是一个位于重复区域的双拷贝基因，编码感染细胞蛋白4（ICP4）。在ICP4中，可以发现EHV-1与EHV-9、EHV-8与EHV-9以及EHV-1与EHV-4之间的重组，前者影响斑马传播的基因型，这是一种感染野生马科动物的EHV-1类型，后者与之前在野外分离株中报道的断点相匹配。因此，这些发现有力地表明ICP4是重组的热点。这项研究描述了新的重组事件，并且是首次使用所有可用的马属甲型疱疹病毒物种基因组进行全基因组重组分析。
