Pagamjav Ochir, Sakata Tohru, Matsumura Tomio, Yamaguchi Tsuyoshi, Fukushi Hideto
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Japan.
Microbiol Immunol. 2005;49(2):167-79. doi: 10.1111/j.1348-0421.2005.tb03716.x.
Equine herpesvirus 1 (EHV-1) is a pathogen causing rhinopneumonia in young horses, abortion in mares, and myeloencephalitis in adult horses. Two types, EHV-1 P and EHV-1 B, have recently been dominant among 16 electropherotypes. EHV-1 P and EHV-1 B viruses were compared by long and accurate polymerase chain reaction (LA-PCR) and restriction fragment length polymorphism (RFLP) analysis. Differences in restriction sites were found to be focused in ORF64, which encodes the infected cell protein 4 (ICP4), and downstream of the ICP4 gene. The 3 ' -end and downstream of ICP4 gene of EHV-1 B were found to be replaced by the corresponding region of EHV-4, indicating that EHV-1 B is a naturally occurring recombinant virus between progenitors of EHV-1 P and EHV-4. This is the first report showing a natural interspecies recombinant in alphaherpesviruses.
马疱疹病毒1型（EHV-1）是一种可导致幼马鼻肺炎、母马流产以及成年马脑脊髓炎的病原体。最近，在16种电泳型中，EHV-1 P和EHV-1 B这两种类型占主导地位。通过长片段精确聚合酶链反应（LA-PCR）和限制性片段长度多态性（RFLP）分析对EHV-1 P和EHV-1 B病毒进行了比较。发现限制性酶切位点的差异集中在编码感染细胞蛋白4（ICP4）的ORF64以及ICP4基因的下游。发现EHV-1 B的ICP4基因3'端及下游被EHV-4的相应区域所取代，这表明EHV-1 B是EHV-1 P和EHV-4的祖代之间自然产生的重组病毒。这是首次报道在α疱疹病毒中出现自然种间重组。
