College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China.
Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), Qingdao, 266071, PR China.
Anal Chim Acta. 2023 Oct 16;1278:341688. doi: 10.1016/j.aca.2023.341688. Epub 2023 Aug 4.
In this work, a wearable and flexible three-electrode electrochemical sensing system (TESS) by using a two-in-one enzyme-mimic working electrode (TIOWE) is reported. The integrated three-electrode, including working electrodes, reference electrodes, and counter electrodes are formed by transfer printing of NiP-based composite electrode ink (NiP/G ink), Ag/AgCl ink, and carbon ink onto PDMS substrate, respectively. The NiP/G ink-based working electrodes have both good conductivity and enzyme-mimic catalytic activity towards glucose. Under optimized conditions, the TIOWE-TESS has a low detection limit of 0.37 μM and wide linear ranges of 0.001 mM-0.1 mM and 0.1 mM-1.4 mM. Furthermore, the TIOWE-TESS has good applicability in serum samples and reveals remarkable electrochemical performance at fluctuant working temperatures. The proposed TIOWE-TESS can be integrated on a waterproof bandage to fabricate a skin-friendly patch device for sweet glucose monitoring, which highlights its potential applications in flexible and wearable commercial devices for health-monitoring.
本工作报道了一种基于二合一酶模拟工作电极（TIOWE）的可穿戴柔性三电极电化学传感系统（TESS）。集成的三电极，包括工作电极、参比电极和对电极，分别通过 NiP 基复合电极油墨（NiP/G 油墨）、Ag/AgCl 油墨和碳油墨的转印到 PDMS 基底上形成。基于 NiP/G 油墨的工作电极具有良好的导电性和对葡萄糖的酶模拟催化活性。在优化条件下，TIOWE-TESS 的检测限低至 0.37 μM，线性范围为 0.001 mM-0.1 mM 和 0.1 mM-1.4 mM。此外，TIOWE-TESS 在血清样品中具有良好的适用性，并在波动的工作温度下表现出显著的电化学性能。所提出的 TIOWE-TESS 可以集成到防水绷带上来制造用于监测血糖的皮肤友好型贴片设备，这突出了其在用于健康监测的柔性和可穿戴商业设备中的潜在应用。
