Department of Molecular Medicine, Faculty of Medicine, Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, 9717853577, Iran.
Nanofanavaran partopooyesh Company, Science and Technology Park of South Khorasan, Birjand, 9718643683, Iran.
Sci Rep. 2023 Nov 28;13(1):20898. doi: 10.1038/s41598-023-48322-y.
An unusual strategy was designed to fabricate conductive patterns for flexible surfaces, which were utilized for non-enzymatic amperometric glucose sensors. The Ag/AgCl/Ag quasi-reference ink formulation utilized two reducing agents, NaBH[Formula: see text] and ethylene glycol. The parameters of the ink, such as sintering time and temperature, NaBH[Formula: see text] concentration, and layer number of coatings on flexible laser-induced graphene (LIG) electrodes were investigated. The conductive Ag/AgCl/Ag ink was characterized using electrochemical and surface analysis techniques. The electrocatalytic activity of Ag/AgCl/Ag NRs can be attributed to their high surface area, which offer numerous active sites for catalytic reactions. The selectivity and sensitivity of the electrodes for glucose detection were investigated. The XRD analysis showed (200) oriented AgCl on covered Ag NRs, and with the addition of NaBH[Formula: see text], the intensity of the peaks of the Ag NRs increased. The wide linear range of non-enzymatic sensors was attained from 0.003 to 0.18 mM and 0.37 to 5.0 mM, with a low limit of detection of 10 [Formula: see text]M and 20 [Formula: see text]M, respectively.The linear range of enzymatic sensor in real sample was determined from 0.040 to 0.097 mM with a detection limit of 50 [Formula: see text]M. Furthermore, results of the interference studies demonstrated excellent selectivity of the Ag/AgCl/Ag NRs/LIG electrode. The Ag/AgCl/Ag NRs on the flexible LIG electrode exhibited excellent sensitivity,long-time stablity,and reproducibility. The efficient electroactivity were deemed suitable for various electrochemical applications and biosensors.
设计了一种用于在柔性表面上制造导电图案的独特策略，该策略用于非酶安培葡萄糖传感器。Ag/AgCl/Ag 准参比墨水配方使用了两种还原剂，即 NaBH4 和乙二醇。研究了墨水的参数，如烧结时间和温度、NaBH4 浓度以及柔性激光诱导石墨烯 (LIG) 电极上涂层的层数。使用电化学和表面分析技术对导电 Ag/AgCl/Ag 油墨进行了表征。Ag/AgCl/Ag NRs 的电催化活性可归因于其高表面积，为催化反应提供了众多活性位点。研究了电极对葡萄糖检测的选择性和灵敏度。XRD 分析表明，AgCl 覆盖在 Ag NRs 上具有 (200) 取向，并且随着 NaBH4 的加入，Ag NRs 的峰强度增加。非酶传感器的宽线性范围从 0.003 到 0.18 mM 和 0.37 到 5.0 mM，检测限分别为 10 [Formula: see text]M 和 20 [Formula: see text]M。在实际样品中，酶传感器的线性范围从 0.040 到 0.097 mM，检测限为 50 [Formula: see text]M。此外，干扰研究的结果表明 Ag/AgCl/Ag NRs/LIG 电极具有出色的选择性。柔性 LIG 电极上的 Ag/AgCl/Ag NRs 表现出出色的灵敏度、长时间稳定性和可重复性。高效的电活性被认为适合各种电化学应用和生物传感器。
