Rosenberg M, Paterson B M
Nature. 1979 Jun 21;279(5715):696-701. doi: 10.1038/279696a0.
Certain polycistronic prokaryotic mRNAs, when modified at their 5'-termini with a cap structure, are translated as efficiently as, or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesising system. However, in this case efficient cap-dependent translation is apparently restricted to the 5'-proximal coding sequence. Moreover, certain translational regulatory signals potentially used by these prokaryotic mRNAs to regulate their levels of expression seem to be recognised by the eukaryotic translational components. The evolutionary significance and practical implications of these results are discussed.
某些多顺反子原核mRNA在其5′末端经帽结构修饰后，在真核无细胞蛋白质合成系统中翻译效率与真核mRNA相同，甚至更高。然而，在这种情况下，有效的帽依赖性翻译显然仅限于5′近端编码序列。此外，这些原核mRNA可能用于调节其表达水平的某些翻译调控信号似乎能被真核翻译组分识别。文中讨论了这些结果的进化意义和实际应用。
