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基于二代测序数据的尖吻蝮基因组组装与注释

Genome assembly and annotation of the Sharp-nosed Pit Viper based on next-generation sequencing data.

作者信息

Wang Xinyu, Liu Lirong, Zhu Wenbiao, Wang Shiqing, Shi Minhui, Yang Shuhui, Lu Haorong, Cao Jun

机构信息

State Key Laboratory of Agricultural Genomics, Key Laboratory of Genomics, Ministry of Agriculture, BGI Research, Shenzhen 518083, China.

China National GeneBank, BGI, Shenzhen 518120, China.

出版信息

GigaByte. 2023 Sep 4;2023:gigabyte88. doi: 10.46471/gigabyte.88. eCollection 2023.

Abstract

The study of the currently known >3,000 species of snakes can provide valuable insights into the evolution of their genomes. , also known as Sharp-nosed Pit Viper, one hundred-pacer viper or five-pacer viper, is a venomous snake with significant economic, medicinal and scientific importance. Widely distributed in southeastern China and South-East Asia, has been primarily studied for its venom. Here, we employed next-generation sequencing to assemble and annotate a highly continuous genome of . The genome size is 1.46 Gb; its scaffold N50 length is 6.21 Mb, the repeat content is 42.81%, and 24,402 functional genes were annotated. This study helps to further understand and utilize and its venom at the genetic level.

摘要

对目前已知的3000多种蛇类进行研究,有助于深入了解其基因组的进化。,又称尖吻蝮、百步蛇或五步蛇,是一种具有重要经济、药用和科学价值的毒蛇。广泛分布于中国东南部和东南亚地区,主要因其毒液而受到研究。在此,我们利用二代测序技术对的高度连续基因组进行了组装和注释。该基因组大小为1.46Gb;其支架N50长度为6.21Mb,重复序列含量为42.81%,共注释了24402个功能基因。本研究有助于在基因层面进一步了解和利用及其毒液。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/10498098/2a4839570751/gigabyte-2023-88-g001.jpg

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