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白唇竹叶青蛇的基因组组装与注释

The genome assembly and annotation of the white-lipped tree pit viper .

作者信息

Niu Xiaotong, Lv Yakui, Chen Jin, Feng Yueheng, Cui Yilin, Lu Haorong, Liu Hui

机构信息

Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Ministry of Education), School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou, 570228, China.

State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen, 518083, China.

出版信息

GigaByte. 2024 Jan 25;2024:gigabyte106. doi: 10.46471/gigabyte.106. eCollection 2024.

Abstract

, also known as the white-lipped pit viper or white-lipped tree viper, is a highly venomous snake distributed across Southeast Asia and the cause of many snakebite cases. In this study, we report the first whole genome assembly of obtained with next-generation sequencing from a specimen collected in Mengzi, Yunnan, China. After genome sequencing and assembly, the genome of this male individual was 1.51 Gb in length and included 38.42% repeat-element content. Using this genome, 21,695 genes were identified, and 99.17% of genes could be annotated using gene functional databases. Our genome assembly and annotation process was validated using a phylogenetic tree, which included six species and focused on single-copy genes of nuclear genomes. This research will contribute to future studies on biology and the genetic basis of snake venom.

摘要

(该物种),也被称为白唇竹叶青或白唇树蝰,是一种分布于东南亚的剧毒蛇类,也是许多蛇咬伤病例的起因。在本研究中,我们报告了从中国云南蒙自采集的一个标本中通过下一代测序获得的(该物种)首个全基因组组装结果。经过基因组测序和组装,这个雄性(该物种)个体的基因组长度为1.51Gb,重复元件含量为38.42%。利用这个基因组,鉴定出了21695个基因,并且99.17%的基因可以使用基因功能数据库进行注释。我们的基因组组装和注释过程通过一棵系统发育树进行了验证,该系统发育树包括六个物种,并聚焦于核基因组的单拷贝基因。这项研究将有助于未来对(该物种)生物学和蛇毒遗传基础的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5134/10836062/8dc2478cfa20/gigabyte-2024-106-g002.jpg

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