Department of Pediatrics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, No. 301 Middle Yanchang Road, Shanghai, China.
Institute for Intestinal Diseases, Tongji University School of Medicine, No. 301 Middle Yanchang Road, Shanghai, China.
Curr Microbiol. 2023 Sep 15;80(11):341. doi: 10.1007/s00284-023-03444-4.
Infants born via cesarean section (CS) are at an increased risk of immune-related diseases later in life, potentially due to altered gut microbiota. Recent research has focused on the administration of probiotics in the prevention of gut microbiota dysbiosis in neonates delivered by CS. This study was performed to investigate the effects of probiotic supplementation on the gut microbiota of CS-delivered infants.
Thirty full-term neonates delivered by CS were randomized into the intervention (supplemented orally with a probiotic containing Bifidobacterium longum, Lactobacillus acidophilus, and Enterococcus faecalis for 2 weeks) and control groups. Stool samples were collected at birth and 2 weeks and 42 days after birth. The composition of the gut microbiota was analyzed using 16S rRNA sequencing technology.
The applied bacterial strains were abundant in the CS-delivered infants supplemented with probiotics. Probiotics increased the abundance of some beneficial bacteria, such as Bacteroides, Acinetobacter, Veillonella, and Faecalibacterium. Low colonization of Klebsiella, a potentially pathogenic bacterium, was observed in the intervention group.
Our results showed that probiotics supplemented immediately after CS enriched the gut microbiota composition and altered the pattern of early gut colonization.
registration number NCT05086458.
通过剖宫产（CS）出生的婴儿在以后的生活中患与免疫相关的疾病的风险增加，这可能是由于肠道微生物群的改变。最近的研究集中在通过 CS 分娩的新生儿中使用益生菌来预防肠道微生物群失调。本研究旨在研究益生菌补充对 CS 分娩婴儿肠道微生物群的影响。
30 名足月通过 CS 分娩的新生儿被随机分为干预组（口服补充含有长双歧杆菌、嗜酸乳杆菌和粪肠球菌的益生菌 2 周）和对照组。在出生时、出生后 2 周和 42 天时采集粪便样本。使用 16S rRNA 测序技术分析肠道微生物群的组成。
应用的细菌菌株在接受益生菌补充的 CS 分娩婴儿中丰富。益生菌增加了一些有益细菌的丰度，如拟杆菌属、不动杆菌属、韦荣球菌属和粪杆菌属。干预组中观察到潜在致病菌克雷伯氏菌的定植较低。
我们的结果表明，CS 后立即补充益生菌丰富了肠道微生物群组成，并改变了早期肠道定植的模式。
注册号 NCT05086458。
