Department of Neonatal Diagnosis and Treatment Center, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
Am J Perinatol. 2021 Sep;38(11):1181-1191. doi: 10.1055/s-0040-1710559. Epub 2020 May 23.
The delivery mode is considered to be a significant influencing factor in the early gut microbiota composition, which is associated with the long-term health of the host. In this study, we tried to explore the effects of probiotics on the intestinal microbiota of C-section neonates.
Twenty-six Chinese neonates were enrolled in this study. The neonates were divided into four groups: VD (natural delivery neonates, = 3), CD (cesarean-born neonates, = 9), CDL (cesarean-born neonates supplemented with probiotic at a lower dosage, = 7), and CDH (cesarean-born neonates supplemented with probiotic at a higher dosage, = 7). Fecal samples were collected on the 3rd, 7th, and 28th day since birth. The V3-V4 region of the 16S ribosomal ribonucleic acid gene was sequenced by next-generation sequencing technology.
The α-diversity of the intestinal microbiota of cesarean delivery neonates was significantly lower than that of the naturally delivered neonates on the 28th day ( = 0.005). After supplementation with probiotics for 28 days, the α-diversity and the β-diversity of the gut flora in the cesarean-born infants (CDL28 and CDH28) was similar to that in the vaginally delivery infants. Meanwhile, the abundances of and were significantly increased since the 3rd day of probiotic supplementation. Besides, the sustained supplementation of probiotics to neonates would help improve the abundance of the operational taxonomic units in several different Clusters of Orthologous Groups of proteins.
This study showed that probiotics supplementation to cesarean-born neonates since birth might impact the diversity and function of gut microbiota.
· Cesarean-born neonates. · Probiotic supplementation impact gut flora. · Bifidobacterium and Lactobacillus.
分娩方式被认为是影响早期肠道微生物群组成的重要因素，而肠道微生物群的组成又与宿主的长期健康密切相关。本研究旨在探讨益生菌对剖宫产新生儿肠道微生物群的影响。
本研究纳入了 26 名中国新生儿。将新生儿分为四组：VD（自然分娩新生儿，n=3）、CD（剖宫产新生儿，n=9）、CDL（剖宫产新生儿低剂量补充益生菌，n=7）和 CDH（剖宫产新生儿高剂量补充益生菌，n=7）。分别在出生后第 3、7 和 28 天采集粪便样本。采用下一代测序技术对 16S 核糖体核糖核酸基因的 V3-V4 区进行测序。
剖宫产新生儿肠道微生物群的 α 多样性在第 28 天显著低于自然分娩新生儿（P=0.005）。补充益生菌 28 天后，剖宫产儿（CDL28 和 CDH28）肠道菌群的 α 多样性和β 多样性与阴道分娩儿相似。同时，自益生菌补充第 3 天起，和 的丰度显著增加。此外，持续给新生儿补充益生菌有助于提高几个不同的同源基因操作分类单元的丰度。
本研究表明，从出生开始给剖宫产儿补充益生菌可能会影响肠道微生物群的多样性和功能。
· 剖宫产儿；· 益生菌补充影响肠道菌群；· 双歧杆菌和乳杆菌。
