Department of Botany, Women University Swabi, Swabi, Khyber Pakhtun Khwa, Pakistan.
National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Center Islamabad Pakistan, Islamabad, Capital, Pakistan.
PeerJ. 2023 Sep 12;11:e15832. doi: 10.7717/peerj.15832. eCollection 2023.
Sugarcane is the world's largest cultivated crop by biomass and is the main source of sugar and biofuel. Sucrose phosphate synthase (SPS) enzymes are directly involved in the synthesis of sucrose. Here, we analyzed and compared one of the important gene families involved in sucrose metabolism in a high and low sucrose sugarcane cultivar. A comprehensive analysis of the SoSPS family displayed their phylogenetic relationship, gene and protein structure, miRNA targets, protein interaction network (PPI), gene ontology and collinearity. This was followed by a spatial expression analysis in two different sugarcane varieties. The phylogenetic reconstruction distributed AtSPS, ZmSPS, OsSPS, SoSPS and SbSPS into three main groups (A, B, C). The regulatory region of genes carries , , G-box, and as the most dominant elements. The PPI analysis predicted a total of 14 unique proteins interacting with SPS. The predominant expression of SPS in chloroplast clearly indicates that they are the most active in the organelle which is the hub of photosynthesis. Similarly, gene ontology attributed SPS to sucrose phosphate synthase and glucosyl transferase molecular functions, as well as sucrose biosynthetic and disaccharide biological processes. Overall, the expression of SPS in CPF252 (high sucrose variety) was higher in leaf and culm as compared to that of CPF 251 (low sucrose variety). In brief, this study adds to the present literature about sugarcane, sucrose metabolism and role of SPS in sucrose metabolism thereby opening up further avenues of research in crop improvement.
甘蔗是世界上生物量最大的栽培作物，是糖和生物燃料的主要来源。蔗糖磷酸合酶（SPS）酶直接参与蔗糖的合成。在这里，我们分析并比较了高、低糖甘蔗品种中参与蔗糖代谢的一个重要基因家族。SoSPS 家族的综合分析显示了它们的系统发育关系、基因和蛋白质结构、miRNA 靶标、蛋白质相互作用网络（PPI）、基因本体论和共线性。随后在两个不同的甘蔗品种中进行了空间表达分析。系统发育重建将 AtSPS、ZmSPS、OsSPS、SoSPS 和 SbSPS 分布为三个主要组（A、B、C）。基因的调控区携带 、 、G-盒和 作为最主要的 元件。PPI 分析预测了总共 14 个与 SPS 相互作用的独特蛋白质。SPS 在叶绿体中的主要表达清楚地表明，它们在光合作用的中心细胞器中最为活跃。同样，基因本体论将 SPS 归因于蔗糖磷酸合酶和葡萄糖基转移酶的分子功能，以及蔗糖的生物合成和二糖的生物过程。总的来说，CPF252（高糖品种）叶片和茎中的 SPS 表达高于 CPF251（低糖品种）。总之，这项研究增加了关于甘蔗、蔗糖代谢和 SPS 在蔗糖代谢中的作用的现有文献，从而为作物改良的进一步研究开辟了新的途径。
