Witz G, Lawrie N J, Zaccaria A, Ferran H E, Goldstein B D
J Free Radic Biol Med. 1986;2(1):33-9. doi: 10.1016/0748-5514(86)90121-2.
The standard assay for lipid peroxidation is the measurement of the pink, 532 nm absorbing chromogen which is formed upon reaction of 2-thiobarbituric acid (TBA) with the lipid peroxidation product malonaldehyde (MDA). The present studies indicate that the toxic lipid peroxidation product trans-4-hydroxynonenal and its dehydration product trans,trans-nonadienal react with TBA to form chromogens which absorb maximally at 530 and 532 nm, respectively. Other biologically active alpha,beta-unsaturated aldehydes, such as acrolein and crotonaldehyde, short-chain homologs of alkenals formed during lipid peroxidation, and trans,trans-muconaldehyde, a novel diene dialdehyde, react with TBA to form products which absorb maximally at 495 nm. The molar extinction coefficients of the aldehyde:TBA chromogens formed were found to vary widely, suggesting that only small contributions to the 532 nm absorption by TBA adducts of reactive aldehydes other than MDA may be encountered during the use of the TBA assay.
脂质过氧化的标准检测方法是测量粉红色、在532nm处有吸收的色原，该色原是在2-硫代巴比妥酸（TBA）与脂质过氧化产物丙二醛（MDA）反应时形成的。目前的研究表明，有毒的脂质过氧化产物反式-4-羟基壬烯醛及其脱水产物反式，反式-壬二烯醛与TBA反应形成分别在530nm和532nm处有最大吸收的色原。其他生物活性的α，β-不饱和醛，如丙烯醛和巴豆醛（脂质过氧化过程中形成的烯醛的短链同系物）以及反式，反式-粘康醛（一种新型二烯二醛），与TBA反应形成在495nm处有最大吸收的产物。发现所形成的醛：TBA色原的摩尔消光系数差异很大，这表明在使用TBA检测法时，除MDA外的反应性醛的TBA加合物对532nm吸收的贡献可能很小。
