Knight J A, Pieper R K, McClellan L
Department of Pathology, University of Utah School of Medicine, Salt Lake City.
Clin Chem. 1988 Dec;34(12):2433-8.
The thiobarbituric acid (TBA) reaction, quantified by colorimetry or fluorimetry, is the method most widely used for studying lipid peroxidation in both laboratory animals and in humans with disorders. However, concerns regarding its analytical specificity have often been expressed, because TBA reacts with a wide variety of chemical species to produce a pink to red color. In this study, we reacted TBA with various saturated and unsaturated aldehydes (both directly and in the presence of sucrose, fructose, and glucose), substituted pyrimidines, 2-deoxyribose, and N-acetylneuraminic acid. We also studied the TBA reaction with bilirubin, biliverdin, icteric serum, and serum containing hemolyzed erythrocytes, comparing the absorption spectra of these reaction products with that for malondialdehyde (MDA). The reaction products were also analyzed for MDA by high-performance liquid chromatography (HPLC). Although the TBA reaction with some of these compounds may not be important in biological studies, others could lead to misinterpretations of increased lipid peroxidation. Use of HPLC to quantify MDA is recommended because of its high analytical sensitivity and specificity, especially in the study of lipid peroxidation in human subjects.
硫代巴比妥酸（TBA）反应通过比色法或荧光法进行定量，是在实验动物和患有疾病的人类中研究脂质过氧化最广泛使用的方法。然而，人们经常对其分析特异性表示担忧，因为TBA能与多种化学物质反应产生粉红色至红色。在本研究中，我们使TBA与各种饱和醛和不饱和醛（直接以及在蔗糖、果糖和葡萄糖存在的情况下）、取代嘧啶、2-脱氧核糖和N-乙酰神经氨酸发生反应。我们还研究了TBA与胆红素、胆绿素、黄疸血清和含有溶血红细胞的血清的反应，将这些反应产物的吸收光谱与丙二醛（MDA）的吸收光谱进行比较。还通过高效液相色谱法（HPLC）对反应产物进行MDA分析。尽管TBA与其中一些化合物的反应在生物学研究中可能并不重要，但其他反应可能会导致对脂质过氧化增加的误解。由于其高分析灵敏度和特异性，建议使用HPLC对MDA进行定量，尤其是在人类受试者脂质过氧化的研究中。
