Molecular Detection of spp. in Ticks of Sheep and Goats by Nested PCR Method in West Azerbaijan Province, Iran.

Ticks are an important vector among arthropods associated with serious medical and veterinary problems. In this research, we investigate species in ticks isolated from the surface of livestock (sheep and goat) in different regions of West Azerbaijan province. Polymerase chain reaction (PCR) was performed using specific primers targeting spp. genes. spp. was identified through PCR. The positive PCR products were sent to Pishgam Company for sequencing. Sequenced data were analyzed, and phylogenetic analysis was performed using maximum likelihood method in MEGA V.10. The detection rate of spp. for gene 69 ( = 542; 12.7%; 95%Cl: 10.1%-15.8%), 42 ( = 542; 7.7%; 95%Cl: 5.78%-10.1%) positive on gene and gene 4 ( = 542; 0.74%; 95%Cl: 0.29%-1.88%). These results are the first report in Iran to identify spp. These results of the study showed that the spp. in hard ticks detected by PCR and it was negative to soft ticks. it is important in public health implications at the studied areas.