Department of Microbiology and Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vector Borne Zoonotic Dis. 2023 Dec;23(12):605-614. doi: 10.1089/vbz.2023.0039. Epub 2023 Sep 19.
Ticks are an important vector among arthropods associated with serious medical and veterinary problems. In this research, we investigate species in ticks isolated from the surface of livestock (sheep and goat) in different regions of West Azerbaijan province. Polymerase chain reaction (PCR) was performed using specific primers targeting spp. genes. spp. was identified through PCR. The positive PCR products were sent to Pishgam Company for sequencing. Sequenced data were analyzed, and phylogenetic analysis was performed using maximum likelihood method in MEGA V.10. The detection rate of spp. for gene 69 ( = 542; 12.7%; 95%Cl: 10.1%-15.8%), 42 ( = 542; 7.7%; 95%Cl: 5.78%-10.1%) positive on gene and gene 4 ( = 542; 0.74%; 95%Cl: 0.29%-1.88%). These results are the first report in Iran to identify spp. These results of the study showed that the spp. in hard ticks detected by PCR and it was negative to soft ticks. it is important in public health implications at the studied areas.
蜱虫是与严重医学和兽医问题相关的节肢动物中的重要传播媒介。在这项研究中，我们调查了从东阿塞拜疆省不同地区牲畜（绵羊和山羊）表面分离出的蜱虫中的 种。使用针对 spp.基因的特异性引物进行聚合酶链反应（PCR）。通过 PCR 鉴定出 spp.。将阳性 PCR 产物送到皮什甘公司进行测序。对测序数据进行分析，并在 MEGA V.10 中使用最大似然法进行系统发育分析。 spp.的检出率为基因 69（ = 542；12.7%；95%Cl：10.1%-15.8%）、基因 42（ = 542；7.7%；95%Cl：5.78%-10.1%）和基因 4（ = 542；0.74%；95%Cl：0.29%-1.88%）。这些结果是伊朗首次鉴定出 spp.的报告。研究结果表明，PCR 检测到硬蜱中的 spp.，而软蜱则为阴性。这在研究地区的公共卫生意义重大。
