伊朗东阿塞拜疆省采用巢式 PCR 方法检测猫和狗血液样本中的螺旋体属：分子检测和系统发育分析。
Molecular detection and phylogenetic analysis of Borrelia Spp. In blood samples of cats and dogs by the nested-PCR method in West Azerbaijan Province, Iran.
机构信息
Department of Microbiology, Urmia University, Urmia, Iran.
Department of Internal Medicine and Clinical Pathology, Urmia University, Urmia, Iran.
出版信息
Braz J Microbiol. 2024 Sep;55(3):2915-2922. doi: 10.1007/s42770-024-01401-2. Epub 2024 May 31.
The objective of this study was to investigate the presence and genetic attributes of Borrelia spp. in cats and dogs from the West Azerbaijan Province, located in the northwest of Iran. A total of 250 blood samples from cats and 300 blood samples from dogs were collected, and information regarding their age, sex, breed, ownership status, sampling time and region was recorded. The identification of positive samples was accomplished through nested-PCR and sequencing, with subsequent analysis of the gene sequences conducted using BioEdit software. The gene sequences for Borrelia spp. in this study showed 100% similarity to reference sequences in the GenBank® database. Phylogenetic trees were built using MEGA11. The outcomes indicated that among 250 blood samples from cats, 48 (19.2%) tested positive for Borrelia spp. gene, with a CI from 14.8 to 24.53% for cats. Similarly, out of 300 blood samples from dogs, 45 (15%) tested positive for the Borrelia spp. gene, with a CI from 11.4 to 19.48% for dogs.
本研究旨在调查来自伊朗西北部东阿塞拜疆省的猫和狗中是否存在伯氏疏螺旋体属（Borrelia spp.）及其遗传特征。共采集了 250 份猫的血液样本和 300 份狗的血液样本，并记录了它们的年龄、性别、品种、所有权状况、采样时间和地区信息。通过巢式 PCR 和测序对阳性样本进行鉴定，随后使用 BioEdit 软件对基因序列进行分析。本研究中伯氏疏螺旋体属的基因序列与 GenBank®数据库中的参考序列具有 100%的相似性。使用 MEGA11 构建了系统发育树。结果表明，在 250 份猫的血液样本中，有 48 份（19.2%）检测到伯氏疏螺旋体属基因阳性，猫的置信区间为 14.8%至 24.53%。同样，在 300 份狗的血液样本中，有 45 份（15%）检测到伯氏疏螺旋体属基因阳性，狗的置信区间为 11.4%至 19.48%。
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