Enferadi Ahmad, Ownagh Abdolghaffar, Tavassoli Mousa
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Iran.
Vet Res Forum. 2024;15(2):89-95. doi: 10.30466/vrf.2023.2000526.3855. Epub 2024 Feb 15.
species are spirochetes transmitted by ticks that are important in human and animals. In most countries, there is still no molecular epidemiology of borreliosis in ruminants. This study was aimed to evaluate the existence of spp. DNA in the blood samples of small ruminants using polymerase chain reaction (PCR) method in West Azerbaijan Province, Iran. To detect spp. DNA, about 1,018 ruminants (456 goats and 562 sheep) blood samples were examined from different bioclimatic regions in West Azerbaijan province, Iran. The DNA extracting and PCR were conducted. In sheep, the following prevalence rates were respectively obtained for the and genes: 3.55% (20/562), 2.13% (12/562) and 0.88% (5/562). And so, the prevalence rates of the genes in goats were 0.87% (4/456) for gene, 1.75% (8/456) for gene and 0.65% (3/456) for gene. The prevalence of spp. was significantly different in small ruminants based on the farms and localities. The sheep and goats in humid areas (north of West Azerbaijan) were infected statistically more than those in sub-humid areas (south of West Azerbaijan). It is demonstrated that host species like sheep and goats may have a key role in natural Lyme disease cycles and other borreliosis diseases in Iran.
这些物种是由蜱传播的螺旋体，对人类和动物都很重要。在大多数国家，反刍动物中莱姆病的分子流行病学研究仍属空白。本研究旨在利用聚合酶链反应（PCR）方法评估伊朗西阿塞拜疆省小型反刍动物血液样本中疏螺旋体属物种的DNA存在情况。为检测疏螺旋体属物种的DNA，从伊朗西阿塞拜疆省不同生物气候区域采集了约1018份反刍动物（456只山羊和562只绵羊）的血液样本，并进行了DNA提取和PCR检测。在绵羊中，分别获得了以下基因的流行率：基因A为3.55%（20/562），基因B为2.13%（12/562），基因C为0.88%（5/562）。在山羊中，基因A的流行率为0.87%（4/456），基因B的流行率为1.75%（8/456），基因C的流行率为0.65%（3/456）。基于农场和地区的不同，小型反刍动物中疏螺旋体属物种的流行率存在显著差异。湿润地区（西阿塞拜疆省北部）的绵羊和山羊感染率在统计学上高于半湿润地区（西阿塞拜疆省南部）。结果表明，绵羊和山羊等宿主物种在伊朗自然莱姆病循环及其他疏螺旋体病中可能起着关键作用。
