Ye Yuhan, Xu Xianyin, Mao Bingyong, Tang Xin, Cui Shumao, Zhao Jianxin, Zhang Qiuxiang
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd, Shanghai 200436, China.
Food Funct. 2023 Mar 20;14(6):2847-2856. doi: 10.1039/d2fo02938c.
Periodontitis is a chronic inflammatory disease induced by subgingival microbial dysbiosis, characterised by inflammation of the soft tissues of the periodontium and progressive loss of alveolar bone. CCFM1139 is a probiotic with the potential to relieve periodontitis and . Due to the cost of active strain in production applications, we considered the effectiveness of bacterial components and metabolites in alleviating experimental periodontitis. Therefore, this study investigated the effect of heat-inactivated CCFM1139 and its supernatant in the development of experimental periodontitis through animal experiments. The results showed that active, heat-inactivated CCFM1139 and its supernatant all significantly reduced IL-1β levels in gingival tissue and serum ( < 0.05). Micro-computed tomography (micro CT) analysis showed that the active and heat-inactivated CCFM1139 reduced alveolar bone loss in rats with periodontitis by 25.6% and 15.9% respectively ( < 0.05), with no change in percentage of bone volume ( > 0.05). In histomorphometric analysis, active CCFM1139 showed better results in reducing alveolar bone loss and reducing inflammatory cell recruitment at the second molar. In addition, there was no significant difference in the number of tartrate-resistant acid phosphatase (TRAP) positive cells after in all experimental groups ( > 0.05). Therefore, heat-inactivated CCFM1139 or its supernatant also have the ability to relieve periodontitis, and their alleviating effect may focus on the regulation of inflammatory response.
牙周炎是一种由龈下微生物群落失调引起的慢性炎症性疾病，其特征为牙周软组织炎症和牙槽骨的渐进性丧失。CCFM1139是一种有潜力缓解牙周炎的益生菌。由于生产应用中活性菌株的成本问题，我们考虑了细菌成分和代谢产物在减轻实验性牙周炎方面的有效性。因此，本研究通过动物实验探讨了热灭活的CCFM1139及其上清液在实验性牙周炎发展过程中的作用。结果表明，活性、热灭活的CCFM1139及其上清液均显著降低了牙龈组织和血清中的IL-1β水平（P<0.05）。微计算机断层扫描（micro CT）分析显示，活性和热灭活的CCFM1139分别使牙周炎大鼠的牙槽骨丧失减少了25.6%和15.9%（P<0.05），骨体积百分比无变化（P>0.05）。在组织形态计量学分析中，活性CCFM1139在减少第二磨牙牙槽骨丧失和减少炎症细胞募集方面显示出更好的效果。此外，所有实验组中抗酒石酸酸性磷酸酶（TRAP）阳性细胞数量无显著差异（P>0.05）。因此，热灭活的CCFM1139或其上清液也具有缓解牙周炎的能力，其缓解作用可能集中在炎症反应的调节上。
