State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Boroondara, VIC, 3122, Australia.
Adv Healthc Mater. 2021 Oct;10(20):e2100918. doi: 10.1002/adhm.202100918. Epub 2021 Jul 8.
Developing an excellent hemostatic material with good biocompatibility and high blood absorption capacity for rapid hemostasis of deep non-compressible hemorrhage remains a significant challenge. Herein, a novel conjugate electrospinning strategy to prepare an ultralight 3D gelatin sponge consisting of continuous interconnected nanofibers. This unique fluffy nanofiber structure endows the sponge with low density, high surface area, compressibility, and ultrastrong liquid absorption capacity. In vitro assessments show the gelatin nanofiber sponge has good cytocompatibility, high cell permeability, and low hemolysis ratio. The rat subcutaneous implantation studies demonstrate good biocompatibility and biodegradability of gelatin nanofiber sponge. Gelatin nanofiber sponge aggregates and activates platelets in large quantities to accelerate the formation of platelet embolism, and simultaneously escalates other extrinsic and intrinsic coagulation pathways, which collectively contribute to its superior hemostatic capacity. In vivo studies on an ear artery injury model and a liver trauma model of rabbits demonstrate that the gelatin nanofiber sponge rapidly induce stable blood clots with least blood loss compared to gelatin nanofiber membrane, medical gauze, and commercial gelatin hemostatic sponge. Hence, the gelatin nanofiber sponge holds great potential as an absorbable hemostatic agent for rapid hemostasis.
开发一种具有良好生物相容性和高血液吸收能力的优秀止血材料，用于快速止血深部不可压缩性出血，仍然是一个重大挑战。在此，提出了一种新颖的复合电纺策略，用于制备由连续互穿纳米纤维组成的超轻 3D 明胶海绵。这种独特的蓬松纳米纤维结构赋予海绵低密度、高表面积、可压缩性和超强的液体吸收能力。体外评估表明，明胶纳米纤维海绵具有良好的细胞相容性、高细胞通透性和低溶血率。大鼠皮下植入研究表明明胶纳米纤维海绵具有良好的生物相容性和可生物降解性。明胶纳米纤维海绵大量聚集和激活血小板，加速血小板栓塞的形成，同时上调其他外在和内在的凝血途径，共同促成其卓越的止血能力。在兔耳动脉损伤模型和肝创伤模型的体内研究中，与明胶纳米纤维膜、医用纱布和商业明胶止血海绵相比，明胶纳米纤维海绵可迅速诱导稳定的血栓形成，出血量最少。因此，明胶纳米纤维海绵作为一种可吸收的止血剂，具有快速止血的巨大潜力。
