Basiri Parviz, Afshar Saeid, Amini Razieh, Soltanian Ali Reza, Saidijam Massoud, Mahdavinezhad Ali
School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Research Center for Molecular Medicine, Department of Molecular Medicine and Genetics, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran.
Int J Mol Cell Med. 2022;11(4):334-345. doi: 10.22088/IJMCM.BUMS.11.4.334.
MicroRNAs (miRNAs) have emerged as essential gene expression regulators associated with human diseases such as colorectal cancer (CRC). The purpose of this study was to evaluate the expression of miR-330-3p and its target gene BMI1 in tissue samples of patients with CRC, polyp, and healthy adjacent tissue samples and their association with clinicopathological and demographic factors such as agetumor stage, grade, and lymph node invasion of the tumor. Following the extraction of total RNA from approximately 50 mg of colon and rectum tissue of 82 patients with CRC, 13 polypoid lesions, and 26 marginal healthy tissues using RiboEx reagent, cDNA synthesis was performed, and then quantitative real-time PCR was used to detect the expression levels of miR-330-3p and BMI1. Alterations in the gene expression were assessed using the 2 method. The expression of miR-330-3p in all of the CRC samples was significantly lower than in adjacent healthy tissues and polyp (P<0.001). BMI1 was up-regulated in 97.9% of CRC tissue compared to healthy adjacent tissues and polyps (P<0.001). A negative reverse correlation between the miR-330-3p and BMI1 gene was observed in the CRC samples (r= -0.882, P<0.001). Down-regulation of miR-330-3p and BMI1 overexpression strongly correlates with higher tumor stage and lymph node invasion. The AUC for miR-330-3p and BMI1expression was 0.982 (sensitivity, 98.5%; specificity, 78.8%), and 0.971 (sensitivity, 97.6%; specificity, 84.6%) (P<0.001), respectively. Our results indicated that miR-330-3p and BMI1 expression probably could be considered potential diagnostic or prognostic biomarkers for CRC patient.
微小RNA（miRNA）已成为与人类疾病如结直肠癌（CRC）相关的重要基因表达调节因子。本研究的目的是评估miR-330-3p及其靶基因BMI1在CRC患者、息肉患者和健康邻近组织样本中的表达情况，以及它们与临床病理和人口统计学因素如年龄、肿瘤分期、分级和肿瘤淋巴结浸润的关系。使用RiboEx试剂从82例CRC患者、13例息肉样病变和26例边缘健康组织的约50mg结肠和直肠组织中提取总RNA后，进行cDNA合成，然后使用定量实时PCR检测miR-330-3p和BMI1的表达水平。使用2−ΔΔCT方法评估基因表达的变化。所有CRC样本中miR-330-3p的表达均显著低于邻近健康组织和息肉（P<0.001）。与健康邻近组织和息肉相比，97.9%的CRC组织中BMI1上调（P<0.001）。在CRC样本中观察到miR-330-3p与BMI1基因呈负相关（r = -0.882，P<0.001）。miR-330-3p的下调和BMI1的过表达与更高的肿瘤分期和淋巴结浸润密切相关。miR-330-3p和BMI1表达的曲线下面积（AUC）分别为0.982（敏感性，98.5%；特异性，78.8%）和0.971（敏感性，97.6%；特异性，84.6%）（P<0.001）。我们的结果表明，miR-330-3p和BMI1的表达可能被视为CRC患者潜在的诊断或预后生物标志物。
