Ali Akbar-Esfahani Soheila, Karimipoor Morteza, Bahreini Fatemeh, Soltania Ali Reza, Aletaha Najme, Mahdavinezhad Ali
Department of Molecular Medicine & Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Int J Mol Cell Med. 2019 Fall;8(4):240-247. doi: 10.22088/IJMCM.BUMS.8.4.240.
Long non-coding RNAs (lncRNAs), associated with various cancers including colorectal cancer (CRC), could be collected from body fluids easily. Our aims were to determine the expression level of lncRNA in plasma samples of healthy individuals and CRC patients as well as their relationship with clinico-pathological characteristics of patients. First, total RNA was extracted from the plasma samples of 100 subjects including 50 patients and 50 age and sex matched healthy persons. Then, gene expression was measured using real-time PCR technique. The sensitivity and specificity of dysregulation in CRC and healthy individual's plasma was measured by receiver operating characteristic (ROC) analysis. As compared with healthy controls, lncRNA was over expressed in a statistically significant manner in plasma samples of patients (P=0.001). Significant relationship between expression and positive family history of CRC was observed, too (P=0.04). The ROC curve analysis showed an AUC value of 0.775, a specificity of 82%, a sensitivity of 76%, with a cut off value equal to 2.40 (P =0.001). transcript can be proposed as a new biomarker for early diagnosis due to the increased expression in plasma samples of patients with CRC and the relatively high sensitivity and specificity.
长链非编码RNA（lncRNAs）与包括结直肠癌（CRC）在内的多种癌症相关，且能轻松从体液中获取。我们的目的是确定lncRNA在健康个体和CRC患者血浆样本中的表达水平，以及它们与患者临床病理特征的关系。首先，从100名受试者的血浆样本中提取总RNA，其中包括50名患者和50名年龄及性别匹配的健康人。然后，使用实时PCR技术测量基因表达。通过受试者操作特征（ROC）分析来测量CRC患者和健康个体血浆中lncRNA失调的敏感性和特异性。与健康对照相比，lncRNA在患者血浆样本中以具有统计学意义的方式过度表达（P = 0.001）。还观察到lncRNA表达与CRC阳性家族史之间存在显著关系（P = 0.04）。ROC曲线分析显示，曲线下面积（AUC）值为0.775，特异性为82%，敏感性为76%，截断值等于2.40（P = 0.001）。由于CRC患者血浆样本中lncRNA表达增加以及相对较高的敏感性和特异性，该转录本可被提议作为早期诊断的新生物标志物。
