Latvian Biomedical Research and Study Centre, Ratsupites street 1, Riga, 1067, Latvia.
University of Latvia, Jelgavas street 1, Riga, 1004, Latvia.
Biometals. 2024 Feb;37(1):267-274. doi: 10.1007/s10534-023-00538-1. Epub 2023 Sep 20.
Bacterial microcompartments (BMCs) are prokaryotic organelles involved in several biochemical processes in bacterial cells. These cellular substructures consist of an icosahedral shell and an encapsulated enzymatic core. The outer shells of BMCs have been proposed as an attractive platform for the creation of novel nanomaterials, nanocages, and nanoreactors. In this study, we present a method for functionalizing recombinant GRM2-type BMC shell lumens with short cysteine-containing sequences and demonstrate that the iron and cobalt loading capacity of such modified shells is markedly increased. These results also imply that a passive flow of cobalt and iron atoms across the BMC shell could be possible.
细菌微室（BMCs）是参与细菌细胞中几种生化过程的原核细胞器。这些细胞亚结构由二十面体壳和包裹的酶核心组成。BMC 的外壳已被提议作为创建新型纳米材料、纳米笼和纳米反应器的有吸引力的平台。在这项研究中，我们提出了一种用含有短半胱氨酸序列的功能化重组 GRM2 型 BMC 壳腔的方法，并证明了这种修饰壳的铁和钴负载能力显著增加。这些结果还表明，钴和铁原子可能可以通过 BMC 壳的被动流动。
