LncRNA LINC01123 通过靶向 hsa-miR-516b-5p/VEGFA 促进卵巢癌的恶性转化。
LncRNA LINC01123 promotes malignancy of ovarian cancer by targeting hsa-miR-516b-5p/VEGFA.
机构信息
Department of Gynaecology, The Third Affiliated Hospital of Qiqihar Medical University, No. 27, Taishun Street, Tiefeng, Qiqihar, 161000, Heilongjiang, China.
出版信息
Genes Genomics. 2024 Feb;46(2):231-239. doi: 10.1007/s13258-023-01440-3. Epub 2023 Sep 20.
BACKGROUND
Long non-coding RNAs (lncRNAs) play a critical role in the development of ovarian cancer (OC).
OBJECTIVE
The study aimed to determine the role of LncRNA LINC01123 in OC bio-progression, which is upregulated in OC tissues during OC progression.
METHODS
Bioinformatics methods, GEPIA, and qRT-PCR were used to reveal the level and correlation of LINC01123, hsa-miR-516b-5p, and VEGFA, in OC cell lines. MTT, EdU, TUNEL, and Transwell assays were performed to assess the bioactivity of OC cell. Target sites of LINC01123 and hsa-miR-516b-5p were predicted using Starbase, and the potential linkage points of VEGFA and hsa-miR-516b-5p were predicted using TargetScan. These sites and linkage points were confirmed by double luciferase reporter assay.
RESULTS
LINC01123 was upregulated in OC cell lines and LINC01123 silencing suppressed the proliferation and metastasis of OC cells, but promoted cell apoptosis. hsa-miR-516b-5p was linked to LINC01123 and. VEGFA was downstream of hsa-miR-516b-5p. Importantly, silencing of hsa-miR-516b-5p reversed the inhibitory impact of si-LINC01123. The result of hsa-miR-516b-5p inhibitor + si-LINC01123 co-transfection were rescued by si-VEGFA.
CONCLUSION
LINC01123 promotes OC development by dampening miR-516b-5p function, and may be a novel target for treating OC.
背景
长链非编码 RNA（lncRNA）在卵巢癌（OC）的发展中起着关键作用。
目的
本研究旨在确定 LncRNA LINC01123 在 OC 生物进展中的作用，该基因在 OC 组织中上调。
方法
采用生物信息学方法、GEPIA 和 qRT-PCR 揭示 OC 细胞系中 LINC01123、hsa-miR-516b-5p 和 VEGFA 的水平和相关性。MTT、EdU、TUNEL 和 Transwell 测定用于评估 OC 细胞的生物活性。使用 Starbase 预测 LINC01123 和 hsa-miR-516b-5p 的靶位点，使用 TargetScan 预测 VEGFA 和 hsa-miR-516b-5p 的潜在连接点。通过双荧光素酶报告基因测定验证这些位点和连接点。
结果
LINC01123 在 OC 细胞系中上调，LINC01123 沉默抑制 OC 细胞的增殖和转移，但促进细胞凋亡。hsa-miR-516b-5p 与 LINC01123 相关联，VEGFA 是 hsa-miR-516b-5p 的下游靶点。重要的是，沉默 hsa-miR-516b-5p 逆转了 si-LINC01123 的抑制作用。hsa-miR-516b-5p 抑制剂+si-LINC01123 共转染的结果被 si-VEGFA 挽救。
结论
LINC01123 通过抑制 miR-516b-5p 功能促进 OC 发展，可能是治疗 OC 的新靶点。
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