Impact of backslopping in TERASI manufacture, as an improving method to reduce shrimp allergenicity.

The backslopping method was applied to the manufacture of Terasi (fermented shrimp paste) as countermeasures against the technical issue depending on the raw material, and its contribution to reduction of the potential allergenicity of Terasi was examined. Three kinds of starters, the low allergenic Indonesian commercial Terasi (CT), Akiami (Acetes japonicus) Terasi produced in laboratory scale (AT) and heated AT (HAT) were added to the manufacturing process of the Isazaami (Neomysis awatchensis) Terasi which contained tropomyosin (TM) with high IgE-binding ability. Addition of the starter effectively promoting the degradation of the shrimp protein and the reduction of the IgE-binding ability of TM. Interestingly, Terasi added as a starter would have acted primarily as a nutrient source to promote microbial fermentation rather than as a source of fermenting microorganisms and endogenous proteases. The backslopping method is one of the improving methods for reduction of potential allergenicity of Terasi product.