Obermaier Lisa, da Mata Barbara Paes Miglioli, Perego Caio Humberto, Sivieri Kátia, Salgaço Mateus Kawata, Dos Santos André Gonzaga, Boehni Ruth, Groehn Viola, Knapp Jean-Pierre, Rychlik Michael
Chair of Analytical Food Chemistry, Technical University of Munich, Munich, Germany.
Laboratory of Pharmacognosy, School of Pharmaceutical Sciences, São Paulo State University, São Paulo, Brazil.
Front Nutr. 2023 Sep 5;10:1252497. doi: 10.3389/fnut.2023.1252497. eCollection 2023.
Brazil has a diverse plant community, including underutilized non-conventional food crops (PANCs), which have the potential to be a rich source of food and contribute to food security. For assessing the folate content in a range of Brazilian PANCs, we extended the validation of an existing stable isotope dilution assay (SIDA) for the stably C-labelled 10-formyl-Pte[C]Glu (10-CHO-Pte[C]Glu). The SIDA method with an enzymatic treatment, purification step, and an LC-MS/MS measurement was validated regarding linearity, precision, LoD/LoQ, and recovery for 10-CHO-PteGlu. After successful validation, the study of some underutilized Brazilian non-conventional fruits and leaves from the São Paulo State University campus revealed them as an important source of folates. It provided the first insights into the folate content of unexploited food sources from Brazil. Pequi had the highest folate content among the fruits studied, with mean values of 333 μg/100 g based on fresh weight (FW). The analysis also shows that different cultivars of fruit or fruits from different growing locations have a high variability in folate content or other nutritional factors. In most fruits, the main vitamer was 5-CH-Hfolate, but jenipapo and taioba showed the highest content of 10-CHO-PteGlu with 28.22 μg/100 g (FW) in jenipapo peel and 75.64 μg/100 g (FW) in the taioba leaves. Thus, this study also provides results on the importance of the folate vitamer 10-CHO-PteGlu contributing to the total folate content.
巴西拥有多样化的植物群落，包括未充分利用的非传统食用作物（PANCs），这些作物有可能成为丰富的食物来源并有助于粮食安全。为了评估一系列巴西PANCs中的叶酸含量，我们扩展了一种现有的稳定同位素稀释法（SIDA）的验证，该方法用于稳定碳标记的10-甲酰基-Pte[C]Glu（10-CHO-Pte[C]Glu）。对经过酶处理、纯化步骤以及LC-MS/MS测量的SIDA方法进行了线性、精密度、检测限/定量限和10-CHO-PteGlu回收率方面的验证。成功验证后，对圣保罗州立大学校园里一些未充分利用的巴西非传统水果和叶子的研究表明，它们是叶酸的重要来源。该研究首次揭示了巴西未开发食物来源中的叶酸含量。在所研究的水果中，腰果梨的叶酸含量最高，以鲜重（FW）计平均值为333μg/100g。分析还表明，不同品种的水果或来自不同种植地点的水果在叶酸含量或其他营养因素方面存在很大差异。在大多数水果中，主要的维生素形式是5-CH-H叶酸，但人心果和太巴叶显示10-CHO-PteGlu含量最高，人心果果皮中为28.22μg/100g（FW），太巴叶中为75.64μg/100g（FW）。因此，本研究还提供了关于叶酸维生素形式10-CHO-PteGlu对总叶酸含量贡献的重要性的结果。
