Frazão Deborah Ribeiro, Santos Mendes Paulo Fernando, Baia-da-Silva Daiane Claydes, Mendonça de Moura João Daniel, Neves Dos Santos Vinicius Ruan, Matos-Sousa José Mario, de Souza Balbinot Gabriela, Guimarães Douglas Magno, Collares Fabrício Mezzomo, Lima Rafael Rodrigues
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Brazil.
Dental Material Laboratory, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Front Physiol. 2023 Sep 5;14:1214990. doi: 10.3389/fphys.2023.1214990. eCollection 2023.
This study aimed to investigate if apical periodontitis in different periods changes systemic levels of the antioxidant and pro-oxidant parameters in Wistar rats. Twenty-four rats were randomly allocated into healthy animals, apical periodontitis at 14 days (AP14) and apical periodontitis at 28 days (AP28). The first mandibular molars were accessed in the AP groups, and the pulp chamber was exposed to the oral environment, inducing the apical lesion. After 14 and 28 days, the animals were anesthetized, euthanized, and hemimandibles were collected for micro-computed tomography (micro-CT) analysis to measure lesion volume, bone volume (BV), percent of bone to total tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular space (Tb.Sp). A histological examination of the remaining bone was also performed. Finally, blood samples were collected for oxidative biochemistry analysis, investigating glutathione (GSH), Trolox equivalent antioxidant capacity (TEAC), and lipid peroxidation (TBARS). The lesion volume was greater at 28 than at 14 days, as shown by micro-CT. AP14 and AP28 had decreased BV and Tb.Th, but only AP28 showed a reduction in BV/TV. Tb.N and Tb. Sp were increased in apical periodontitis at 28 days. In the histopathological analysis, AP14 had focal regions of moderate mononuclear inflammatory infiltrate, and AP28 had an intense inflammatory infiltrate with bacterial colonies. In the biochemical evaluation, GSH, TEAC, and TBARS were increased after 14 days. However, GSH returned to control levels, TEAC was similar to AP14, and TBARS increased significantly after 28 days. Therefore, the oxidative biochemistry response was modulated according to the progression of periapical damage. After 14 days, the organism could still react to the injury. However, at 28 days, the antioxidant response decreased, associated with an increase in TBARS.
本研究旨在调查不同时期的根尖周炎是否会改变Wistar大鼠体内抗氧化和促氧化参数的系统水平。24只大鼠被随机分为健康组、14天根尖周炎组（AP14）和28天根尖周炎组（AP28）。在AP组中打开第一下颌磨牙，使髓腔暴露于口腔环境，诱发根尖病变。14天和28天后，将动物麻醉、安乐死，并收集半侧下颌骨进行微型计算机断层扫描（micro-CT）分析，以测量病变体积、骨体积（BV）、骨体积占总组织体积的百分比（BV/TV）、小梁厚度（Tb.Th）、小梁数量（Tb.N）和小梁间隙（Tb.Sp）。还对剩余骨进行了组织学检查。最后，采集血样进行氧化生物化学分析，检测谷胱甘肽（GSH）、Trolox等效抗氧化能力（TEAC）和脂质过氧化（TBARS）。如micro-CT所示，28天时的病变体积大于14天时。AP14和AP28的BV和Tb.Th降低，但只有AP28的BV/TV降低。28天根尖周炎时Tb.N和Tb.Sp增加。在组织病理学分析中，AP14有中度单核炎性浸润的局灶区域，AP28有伴有细菌菌落的强烈炎性浸润。在生化评估中，14天后GSH、TEAC和TBARS增加。然而，GSH恢复到对照水平，TEAC与AP14相似，28天后TBARS显著增加。因此，氧化生物化学反应根据根尖周损伤的进展而调节。14天后，机体仍能对损伤作出反应。然而，在28天时，抗氧化反应降低，同时TBARS增加。
