College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.
Hunan Engineering Laboratory for Pollution Control and Waste, Utilization in Swine Production, Changsha, 410128, China.
Curr Microbiol. 2023 Sep 21;80(11):346. doi: 10.1007/s00284-023-03453-3.
Endophytes play essential roles in plant growth under metal(loid)s stress. An endophytic fungus strain MR1 was isolated from the roots of Miscanthus floridulus collected from a lead-zinc mining area (Huayuan, China), which could produce indole-3-acetic acid and have Cadmium (Cd) tolerance. Further 18S rRNA sequencing analysis showed that it was highly similar (99.83%) to Talaromyces pinophilus. In pot experiments, we explored the effects of strain MR1 on the growth and Cd uptake of a wide-type Arabidopsis thaliana under low (LC) and high (HC) Cd concentrations. The results showed that MR1 effectively increased the dry weight of aboveground and underground tissues by 25.95-107.21% in both LC and HC groups. Due to MR1 inoculation, the Cd content in the underground tissues was significantly (p < 0.05) decreased by 39.28% under low Cd concentration, while it was significantly (p < 0.05) increased by 28.28% under high Cd concentration. Besides, MR1 inoculations significantly (p < 0.05) increased the total content of removed Cd (17.080 μg) and BCF (0.064) by 129.77% and 153.95% under high Cd concentration. Therefore, we speculated that MR1 might be selected as the effective microbial agent to increase crop yield and control Cd content in the crop in light Cd-contaminated soil. Besides, MR1 could potentially enhance the phytoremediation efficiency of extremely Cd-contaminated soil.
内生菌在金属（类）胁迫下的植物生长中发挥着重要作用。从来自中国华源铅锌矿区的荻根部分离到一株内生真菌 MR1，它能够产生吲哚-3-乙酸，并且具有镉耐受性。进一步的 18S rRNA 测序分析表明，它与拟青霉（Talaromyces pinophilus）高度相似（99.83%）。在盆栽实验中，我们研究了菌株 MR1 对低（LC）和高（HC）镉浓度下野生型拟南芥生长和镉吸收的影响。结果表明，MR1 在 LC 和 HC 组中分别有效地将地上和地下组织的干重增加了 25.95-107.21%。由于 MR1 的接种，在低镉浓度下，地下组织中镉的含量显著（p < 0.05）降低了 39.28%，而在高镉浓度下则显著（p < 0.05）增加了 28.28%。此外，MR1 的接种显著（p < 0.05）增加了总去除镉量（17.080μg）和 BCF（0.064），在高镉浓度下分别增加了 129.77%和 153.95%。因此，我们推测 MR1 可能被选为有效的微生物剂，以增加作物产量并控制轻度镉污染土壤中作物的镉含量。此外，MR1 可能潜在地提高对极端镉污染土壤的植物修复效率。
