各种预混型糊剂型生物活性陶瓷骨水泥的化学机械性能和生物相容性。

Chemomechanical Properties and Biocompatibility of Various Premixed Putty-type Bioactive Ceramic Cements.

机构信息

Department of Conservative Dentistry, College of Dentistry, Dankook University, Cheonan, Korea.

Department of Conservative Dentistry, School of Dentistry, Dental Research Institute, Dental and Life Science Institute, Pusan National University, Yangsan, Korea.

出版信息

J Endod. 2023 Dec;49(12):1713-1721. doi: 10.1016/j.joen.2023.09.005. Epub 2023 Sep 19.

DOI:10.1016/j.joen.2023.09.005

PMID:37734586

Abstract

INTRODUCTION

This study aimed to evaluate the chemomechanical properties and biocompatibility of recently introduced premixed putty-type bioactive ceramic cements (PPBCs).

METHODS

Including ProRoot MTA (PMTA) as a control, BC RRM fast-set putty (BCPT), Well-Root PT (WRPT), One-Fil PT (OFPT), and Endocem MTA premixed (ECPM) were compared to evaluate setting time, radiopacity, pH change, and microhardness. Biocompatibility on human dental pulp cells was compared using CCK-8 assay. Mineralization potential was evaluated using alkaline phosphatase activity, Alizarin Red S (ARS) staining, and quantitative real-time polymerase chain reaction with odontogenic gene marker. For data analysis, 1-way analysis of variance and Tukey's post hoc test were used at the significance level of 95%.

RESULTS

Among the PPBCs, BCPT presented the longest (552 ± 27) setting time (minutes) and others showed significantly shorter time than PMTA (334 ± 22) (P < .05). WRPT (6.20 ± 0.54) and OFPT (5.82 ± 0.50) showed significantly higher radiopacity values (mmAl) and others showed similar value compared with PMTA (P > .05). All PPBCs showed high alkaline pH from fresh materials and tended to increase according to time periods from 30 minutes to 12 hours. ECPM showed the highest value of microhardness (81.62 ± 5.90), WRPT showed similar, and others showed lower than PMTA (P < .05). All PPBCs showed biocompatibility in CCK-8 assay. All PPBCs showed similar or better value compared with PMTA in ALP and ARS staining, and ALP and DSPP marker expression (P < .05).

CONCLUSIONS

The PPBCs showed clinically acceptable chemomechanical properties and favorable mineralization potential.

摘要

简介

本研究旨在评估最近引入的预混糊剂型生物活性陶瓷水泥（PPBC）的化学机械性能和生物相容性。

方法

包括 ProRoot MTA（PMTA）作为对照，BC RRM 快速凝固糊剂（BCPT）、Well-Root PT（WRPT）、One-Fil PT（OFPT）和 Endocem MTA 预混物（ECPM），以评估凝固时间、射线不透性、pH 值变化和显微硬度。使用 CCK-8 测定法比较对人牙髓细胞的生物相容性。使用碱性磷酸酶活性、茜素红 S（ARS）染色和牙源性基因标志物的定量实时聚合酶链反应评估矿化潜力。对于数据分析，在 95%的置信水平下使用单向方差分析和 Tukey 事后检验。

结果

在 PPBC 中，BCPT 表现出最长的（552±27）凝固时间（分钟），而其他 PPBC 则比 PMTA（334±22）显著缩短（P<.05）。WRPT（6.20±0.54）和 OFPT（5.82±0.50）显示出明显更高的射线不透性值（mmAl），而其他 PPBC 与 PMTA 相比显示出相似的值（P>.05）。所有 PPBC 从新鲜材料开始就呈现出高碱性 pH 值，并随着从 30 分钟到 12 小时的时间段而趋于增加。ECPM 显示出最高的显微硬度值（81.62±5.90），WRPT 显示相似，而其他 PPBC 则低于 PMTA（P<.05）。所有 PPBC 在 CCK-8 测定中均表现出生物相容性。所有 PPBC 在 ALP 和 ARS 染色以及 ALP 和 DSPP 标志物表达方面均显示出与 PMTA 相似或更好的值（P<.05）。