Department of Prosthodontics, School and Hospital of Stomatology, Jilin University, 1500 Qinghua Road, Chaoyang District, Changchun City, Jilin Province, China.
Zuosen Shi, State Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Road, Gaoxin District, Changchun City, Jilin Province, China.
Clin Oral Investig. 2024 Aug 26;28(9):500. doi: 10.1007/s00784-024-05892-z.
To investigate urushiol's potential as a dentin cross-linking agent, promoting remineralization of etched dentin and preventing activation of endogenous proteases causing collagen degradation within the hybrid layer. The goal is to improve bond strength and durability at the resin-dentin interface.
Urushiol primers with varying concentrations were prepared using ethanol and dimethyl sulfoxide (DMSO) as solvents. Dentin from healthy molars underwent grinding and acid etching for 15 s, followed by a 1min application of urushiol primer. After 14 and 28 days of remineralization incubation and remineralization were used to assess by Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR), Micro-Raman spectroscopy, X-ray Diffraction (XRD), Atomic Force Microscopy (AFM), Vickers Hardness, Scanning Electron Microscopy (SEM), and Energy X-ray dispersive spectroscopy (EDS). The overall performance of urushiol primers as dentin adhesives was observed by microtensile bond strength (μTBS) testing and nanoleakage assessment. Investigated the inhibitory properties of the urushiol primers on endogenous metalloproteinases (MMPs) utilizing in situ zymography, and the cytotoxicity of the primers was tested.
Based on ATR-FTIR, Raman, XRD, EM-EDS and Vickers hardness analyses, the 0.7%-Ethanol group significantly enhanced dentin mineral content and improved mechanical properties the most. Pretreatment notably increased the μTBS of restorations, promoted the stability of the mixed layer, and reduced nanoleakage and MMPs activity after 28 days.
The urushiol primer facilitates remineralization in demineralized dentin, enhancing remineralization in etched dentin, effectively improving the bonding interface stability, with optimal performance observed at a 0.7 wt% concentration of the urushiol primer.
研究漆酚作为牙本质交联剂的潜力，促进酸蚀牙本质的再矿化，并防止内源性蛋白酶激活导致混合层内胶原降解。目标是提高树脂-牙本质界面的粘结强度和耐久性。
使用乙醇和二甲基亚砜（DMSO）作为溶剂制备不同浓度的漆酚底漆。取自健康磨牙的牙本质经过研磨和酸蚀 15s，然后用漆酚底漆处理 1min。在再矿化孵育 14 和 28 天后，使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、微拉曼光谱、X 射线衍射（XRD）、原子力显微镜（AFM）、维氏硬度、扫描电子显微镜（SEM）和能量 X 射线能谱（EDS）来评估再矿化情况。通过微拉伸粘结强度（μTBS）测试和纳米渗漏评估观察漆酚底漆作为牙本质粘结剂的整体性能。利用原位酶谱研究漆酚底漆对内源性金属蛋白酶（MMPs）的抑制特性，并测试底漆的细胞毒性。
基于 ATR-FTIR、拉曼、XRD、SEM-EDS 和维氏硬度分析，0.7%-乙醇组显著提高了牙本质的矿物质含量和机械性能。预处理显著提高了修复体的μTBS，促进了混合层的稳定性，并降低了 28 天后的纳米渗漏和 MMPs 活性。
漆酚底漆促进脱矿牙本质的再矿化，增强酸蚀牙本质的再矿化，有效提高粘结界面稳定性，在漆酚底漆浓度为 0.7wt%时表现最佳。
