实验性酸性和中性底涂剂对牙本质生物改性处理对树脂粘结的影响。
Effect of dentin biomodification delivered by experimental acidic and neutral primers on resin adhesion.
机构信息
Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA; Department of Operative Dentistry, College of Dentistry, University São Caetano do Sul, USCS, SP, Brazil.
Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
出版信息
J Dent. 2020 Aug;99:103354. doi: 10.1016/j.jdent.2020.103354. Epub 2020 Apr 30.
OBJECTIVES
Proanthocyanidins (PACs) are biocompounds mimicking native collagen cross-links. The effective and practical delivery of any biocompound is pivotal for clinical usage. The aim was to investigate the dentin biomodification and effective formation of dentin-resin biointerfaces of two highly bioactive PAC-rich extracts, Vitis vinifera (Vv) and Camellia sinensis (Cs), delivered using neutral (NP) or acidic (AP) rinse-out primer approaches.
METHODS
The depth of dentin demineralization (optical profilometry), dentin biomodification (apparent modulus of elasticity, collagen auto-fluorescence) and properties of dentin-resin interfaces (microtensile bond strength - μTBS, and micro-permeability) were investigated. NP consisted of either 15% Vv or Cs applied for 60 s after surface etching; while AP contained 15% Vv or Cs in either 35% glycolic acid or tartaric acid applied for 30 s or 60 s. Data were analyzed using ANOVA and post-hoc tests (α = 0.05).
RESULTS
The depth of demineralization was statistically higher when applied for 60 s, regardless of rinse-out primer approach (p < 0.001). Compared to the AP strategy, NP exhibited statistically higher apparent modulus of elasticity, regardless of PAC extract (p < 0.001). Highest μTBS were obtained for NP, which were statistically similar to AP, when applied for 60 s (p < 0.001); both resulted in a dramatic decrease of the interfacial permeability. NP group showed the lowest μTBS (p < 0.001).
CONCLUSIONS
A combination of high bond strength and low micro-permeability can be accomplished using glycolic acid with the mid- and high-PAC oligomer enriched extract (Vv). Cs extract containing mostly catechins and dimeric PACs, was found unsuitable for resin-dentin adhesion despite exhibiting high initial dentin biomodification.
CLINICAL SIGNIFICANCE
This study provides a new conceptual delivery of PAC-mediated dentin biomodification and conservative dentin surface etching using rinse-out primers. The strategy requires a specific combination of PAC source, α-hydroxy acid, and application time.
目的
原花青素（PACs）是模拟天然胶原交联的生物化合物。任何生物化合物的有效和实际递送对于临床应用都至关重要。本研究旨在通过中性（NP）或酸性（AP）冲洗底漆处理方法，研究两种高生物活性 PAC 丰富提取物——葡萄（Vitis vinifera，Vv）和茶（Camellia sinensis，Cs）的牙本质生物修饰和有效牙本质-树脂生物界面的形成。
方法
通过光学轮廓测量法测量牙本质脱矿深度，通过表观弹性模量和胶原自发荧光评估牙本质生物修饰，通过微拉伸结合强度（μTBS）和微渗透性评估牙本质-树脂界面性能。NP 组分别应用 60s 的 15%Vv 或 Cs 处理牙本质表面，AP 组应用 35%的甘醇酸或酒石酸中 30s 或 60s 的 15%Vv 或 Cs 处理牙本质表面。采用方差分析和事后检验（α=0.05）对数据进行分析。
结果
无论冲洗底漆处理方法如何，60s 处理的脱矿深度均显著更高（p<0.001）。与 AP 策略相比，NP 处理的牙本质表现出更高的表观弹性模量，而 PAC 提取物则没有差异（p<0.001）。NP 处理的 μTBS 最高，60s 处理时与 AP 相似，差异无统计学意义（p<0.001）；两种处理均显著降低了界面渗透性。NP 组的 μTBS 最低（p<0.001）。
结论
使用富含中高 PAC 低聚物的 Vv 与甘醇酸结合，可以实现高结合强度和低微渗透性的组合。尽管 Cs 提取物富含儿茶素和二聚体 PAC，但由于其对树脂-牙本质黏附的不适用性，不适合用于树脂-牙本质黏附。
临床意义
本研究提供了一种使用冲洗底漆处理的 PAC 介导的牙本质生物修饰和保守性牙本质表面蚀刻的新概念输送方法。该策略需要 PAC 源、α-羟基酸和应用时间的特定组合。
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