Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.
School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, Australia.
Front Public Health. 2023 Sep 1;11:1249637. doi: 10.3389/fpubh.2023.1249637. eCollection 2023.
Schistosomiasis, a disease caused by parasites of the genus , remains a global public health threat. This study aimed to validate the diagnostic performance of a recently developed gold immunochromatographic assay (GICA) for the detection of infection in a rural endemic area of the Philippines.
Human clinical samples were collected from 412 subjects living in Laoang and Palapag municipalities, Northern Samar, the Philippines. The presence of -specific antibodies in serum samples was tested with the SjSAP4-incorporated GICA strips and the results were converted to fully quantitative data by introducing an value. The performance of the established GICA was further compared with other diagnostic tools, including the Kato-Katz (KK) technique, point-of-care circulating cathodic antigen (POC-CCA), droplet digital (dd) PCR, and enzyme-linked immunosorbent assays (ELISAs).
The developed GICA strip was able to detect KK positive individuals with a sensitivity of 83.3% and absolute specificity. When calibrated with the highly sensitive faecal ddPCR assay, the immunochromatographic assay displayed an accuracy of 60.7%. Globally, the GICA assay showed a high concordance with the SjSAP4-ELISA assay. The schistosomiasis positivity rate determined by the GICA test was similar to those obtained with the SjSAP4-ELISA assay and the ddPCR assay performed on serum samples (SR_ddPCR), and was 2.3 times higher than obtained with the KK method.
The study further confirms that the developed GICA is a valuable diagnostic tool for detecting light infections and implies that this point-of-care assay is a viable solution for surveying endemic areas of low-intensity schistosomiasis and identifying high-priority endemic areas for targeted interventions.
由 属寄生虫引起的血吸虫病仍然是全球公共卫生威胁。本研究旨在验证一种新开发的金免疫层析检测法（GICA）在菲律宾农村流行地区检测 感染的诊断性能。
从菲律宾北萨马省 Laoang 和 Palapag 市的 412 名居民中采集人临床样本。使用 SjSAP4 整合的 GICA 条带检测血清样本中是否存在 -特异性抗体，并通过引入 值将结果转换为完全定量数据。通过与其他诊断工具（包括加藤厚涂片法（KK）、即时检测循环阴极抗原（POC-CCA）、滴液数字（dd）PCR 和酶联免疫吸附测定（ELISA））比较，进一步评估建立的 GICA 性能。
开发的 GICA 条带能够以 83.3%的灵敏度和绝对特异性检测到 KK 阳性个体。当用高度敏感的粪便 ddPCR 检测法校准时，免疫层析检测法的准确性为 60.7%。总体而言，GICA 检测法与 SjSAP4-ELISA 检测法高度一致。GICA 检测法确定的血吸虫病阳性率与 SjSAP4-ELISA 检测法和血清样本中进行的 SR_ddPCR 检测法获得的阳性率相似，比 KK 法高 2.3 倍。
该研究进一步证实，新开发的 GICA 是一种检测轻度 感染的有价值的诊断工具，并暗示这种即时检测法是调查低强度血吸虫病流行地区和识别重点干预高流行地区的可行解决方案。
