Grasso Giuliana, Sommella Eduardo M, Merciai Fabrizio, Abouhany Rahma, Shinde Sudhirkumar A, Campiglia Pietro, Sellergren Börje, Crescenzi Carlo
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy.
Biofilm Research Center for Biointerfaces, Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 23014, Malmö, Sweden.
Anal Bioanal Chem. 2023 Nov;415(26):6573-6582. doi: 10.1007/s00216-023-04937-8. Epub 2023 Sep 22.
Sphingolipids play crucial roles in cellular membranes, myelin stability, and signalling responses to physiological cues and stress. Among them, sphingosine 1-phosphate (S1P) has been recognized as a relevant biomarker for neurodegenerative diseases, and its analogue FTY-720 has been approved by the FDA for the treatment of relapsing-remitting multiple sclerosis. Focusing on these targets, we here report three novel polymeric capture phases for the selective extraction of the natural biomarker and its analogue drug. To enhance analytical performance, we employed different synthetic approaches using a cationic monomer and a hydrophobic copolymer of styrene-DVB. Results have demonstrated high affinity of the sorbents towards S1P and fingolimod phosphate (FTY-720-P, FP). This evidence proved that lipids containing phosphate diester moiety in their structures did not constitute obstacles for the interaction of phosphate monoester lipids when loaded into an SPE cartridge. Our suggested approach offers a valuable tool for developing efficient analytical procedures.
鞘脂在细胞膜、髓鞘稳定性以及对生理信号和应激的信号反应中发挥着关键作用。其中，鞘氨醇-1-磷酸（S1P）已被公认为神经退行性疾病的相关生物标志物，其类似物FTY-720已获美国食品药品监督管理局（FDA）批准用于治疗复发缓解型多发性硬化症。基于这些靶点，我们在此报告了三种新型聚合物捕获相，用于选择性提取天然生物标志物及其类似物药物。为提高分析性能，我们采用了不同的合成方法，使用了阳离子单体和苯乙烯-二乙烯基苯的疏水共聚物。结果表明，吸附剂对S1P和磷酸芬戈莫德（FTY-720-P，FP）具有高亲和力。这一证据证明，结构中含有磷酸二酯部分的脂质在装入固相萃取柱时，不会对磷酸单酯脂质的相互作用构成障碍。我们建议的方法为开发高效分析程序提供了一个有价值的工具。
