Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Int J Syst Evol Microbiol. 2023 Sep;73(9). doi: 10.1099/ijsem.0.006030.
Obligately anaerobic, Gram-stain-positive, bacilli, strains 12BBH14, 9CFEGH4 and 10CPCBH12, were isolated from faecal samples of healthy Japanese people. Strain 12BBH14 showed the highest 16S rRNA gene sequence similarity to Cla-CZ-80 (97.5 %) and '' Marseille-P3177 (97.2 %). Strain 12BBH14 was also closely related to sp. c-25 with 99.7 % 16S rRNA gene sequence similarity. The 16S rRNA gene sequence analysis showed that strains 12BBH14, 9CFEGH4 and 10CPCBH12 formed a monophyletic cluster with sp. c-25. Near this monophyletic cluster, Cla-CZ-80 and ' Marseille-P3177 formed a cluster and did not form a cluster with other species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 12BBH14, 9CFEGH4, 10CPCBH12 and sp. c-25 were higher than the cut-off values of species demarcation (>88 % dDDH and >98 % ANI), indicating that these four strains are the same species. On the other hand, the dDDH and ANI values of these strains were lower than the cut-off values of species demarcation against other strains (<29 % dDDH and <76 % ANI). Moreover, the average amino acid identity values among these strains were higher than the genus boundary. These results indicate that the isolates should be considered to belong to a new genus of the family . Based on the collected data, strains 12BBH14, 9CFEGH4 and 10CPCBH12 represent a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 12BBH14 (=JCM 35899=DSM 115701). sp. c-25 belongs to . In addition, is transferred to the genus as comb. nov.
严格厌氧菌，革兰氏阳性，杆菌，菌株 12BBH14、9CFEGH4 和 10CPCBH12 从健康日本人的粪便样本中分离得到。菌株 12BBH14 与 Cla-CZ-80（97.5%）和 '' Marseille-P3177（97.2%）的 16S rRNA 基因序列相似度最高。菌株 12BBH14 与 sp. c-25 的 16S rRNA 基因序列相似度也高达 99.7%。16S rRNA 基因序列分析表明，菌株 12BBH14、9CFEGH4 和 10CPCBH12 与 sp. c-25 形成一个单系聚类。在这个单系聚类附近， Cla-CZ-80 和 ' Marseille-P3177 形成一个聚类，与其他 种不形成聚类。菌株 12BBH14、9CFEGH4、10CPCBH12 与 sp. c-25 之间的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值均高于种间区分的临界值（>88% dDDH 和 >98% ANI），表明这 4 株菌为同一物种。另一方面，这些菌株与其他菌株的 dDDH 和 ANI 值低于种间区分的临界值（<29% dDDH 和 <76% ANI）。此外，这些菌株之间的平均氨基酸同一性值高于属的界限。这些结果表明，这些分离株应被认为属于 科的一个新属。基于收集的数据，菌株 12BBH14、9CFEGH4 和 10CPCBH12 代表一个新属的新种，建议将其命名为 属，新种，新种。模式菌株为 12BBH14（=JCM 35899=DSM 115701）。 sp. c-25 属于 。此外， 将 转移到 属中，作为 组合新属。
