Department of Bioinorganic Chemistry, Faculty of Pharmacy, Medical University of Lodz, Lodz, Poland.
Institute of Technology and Food Analysis, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.
Methods Mol Biol. 2024;2717:305-310. doi: 10.1007/978-1-0716-3453-0_20.
Allergies are an ever-increasing health problem in developed societies. Cross-allergies caused by panallergens are a particularly difficult issue. Proteins similar to the main birch pollen Bet v 1 allergen and profilin are some of the most common allergens. These proteins have a very conservative structure and are present in many distinct organisms. Hence, the knowledge of their natural occurrence is very important for the prevention of allergic reactions. The immunometric method is the most useful approach for determining these allergens. The requirement of reliability and simplicity is fulfilled by enzyme-linked immunosorbent assay (ELISA). In this chapter, detailed procedures are described for the determination of Bet v 1 homologous proteins and plant profilins with the use of indirect, noncompetitive ELISA.
过敏是发达社会中日益严重的健康问题。由泛过敏原引起的交叉过敏是一个特别棘手的问题。与主要桦树花粉 Bet v 1 过敏原和丝氨酸蛋白酶相似的蛋白质是一些最常见的过敏原。这些蛋白质具有非常保守的结构，存在于许多不同的生物体中。因此，了解它们的自然存在对于预防过敏反应非常重要。免疫计量法是测定这些过敏原最有用的方法。酶联免疫吸附测定（ELISA）满足了可靠性和简单性的要求。在本章中，详细描述了使用间接、非竞争性 ELISA 测定 Bet v 1 同源蛋白和植物丝氨酸蛋白酶的方法。
