Dutta Koushik, Sarkar Kunal, Karmakar Srikanta, Gangopadhyay Bhuman, Basu Arijita, Bank Sarbashri, De Sriparna, Das Beauty, Das Madhusudan, Chattopadhyay Dipankar
Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
J Mater Chem B. 2023 Oct 11;11(39):9478-9495. doi: 10.1039/d3tb00619k.
Asymmetric scaffolds were developed through electrospinning by utilizing biocompatible materials for effective wound healing applications. First of all, the chitosan surface was modified with decanoyl chloride and crosslinked with collagen to synthesize collagen crosslinked modified-chitosan (CG-cross-CS--Dc). Then, the asymmetric scaffolds were fabricated through electrospinning, where the top layer was a monoaxial nanofiber of the PCL/graphene oxide quantum dot (GOQD) nanocomposite and the bottom layer was a coaxial nanofiber having PCL in the core and the CG-cross-CS--Dc/GOQD nanocomposite in the shell layer. The formation of monoaxial (∼130 ± 50 nm) and coaxial (∼320 ± 40 nm) nanofibers was confirmed by transmission electron microscopy (TEM). The presence of GOQDs contributed to antioxidant and antimicrobial efficacy. These scaffolds showed substantial antibacterial activity against the common wound pathogens and (). The scaffolds exhibited excellent cytocompatibility (MTT assay) and anti-inflammatory behaviour as analysed the cytokine assay and biochemical analysis. The wound healing potential of the nanofibrous scaffolds was assessed with full-thickness excisional wounds in a rat model. The scaffolds accelerated the re-epithelialization as well as the collagen deposition, thereby facilitating the wound healing process in a very short span of time (10 days). Both and analyses thus provide a compelling argument for the use of these scaffolds as therapeutic biomaterials and their suitability for application in rapid wound regeneration and repair.
通过静电纺丝利用生物相容性材料开发了不对称支架，用于有效的伤口愈合应用。首先，用癸酰氯对壳聚糖表面进行改性，并与胶原蛋白交联，以合成胶原蛋白交联改性壳聚糖（CG-cross-CS--Dc）。然后，通过静电纺丝制备不对称支架，其中顶层是聚己内酯/氧化石墨烯量子点（GOQD）纳米复合材料的单轴纳米纤维，底层是一种同轴纳米纤维，其芯部为聚己内酯，壳层为CG-cross-CS--Dc/GOQD纳米复合材料。通过透射电子显微镜（TEM）确认了单轴（130±50nm）和同轴（320±40nm）纳米纤维的形成。GOQDs的存在有助于抗氧化和抗菌功效。这些支架对常见伤口病原体表现出显著的抗菌活性。通过细胞毒性试验（MTT试验）以及细胞因子检测和生化分析表明，这些支架表现出优异的细胞相容性和抗炎行为。在大鼠模型中，用全层切除伤口评估了纳米纤维支架的伤口愈合潜力。这些支架加速了上皮再形成以及胶原蛋白沉积，从而在很短的时间内（10天）促进了伤口愈合过程。因此，细胞毒性试验和细胞因子检测都有力地证明了使用这些支架作为治疗性生物材料以及它们在快速伤口再生和修复中的适用性。
