de Freitas Rayara Nogueira, da Silva Lucas Guilherme Leite, Fiais Gabriela Alice, Ferreira Douglas Sandrac de Biagi, Veras Allice Santos Cruz, Teixeira Giovana Rampazzo, Oliveira Sandra Helena Penha, Dornelles Rita Cássia Menegati, Nakamune Ana Cláudia de Melo Stevanato, Fakhouri Walid D, Chaves-Neto Antonio Hernandes
Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil; Programa de Pós-Graduação em Ciências - Saúde Bucal da Criança, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
Arch Oral Biol. 2023 Nov;155:105805. doi: 10.1016/j.archoralbio.2023.105805. Epub 2023 Sep 16.
To investigate the effects of the anticonvulsant valproic acid (VPA) on salivary glands in male rat using biochemical, functional, histomorphometric, and redox state parameters.
Twenty-four male Wistar rats were randomly distributed into three groups (n = 8 per group): Control (0.9% saline solution), VPA100 (100 mg/kg), and VPA400 (400 mg/kg). After 21 consecutive days of treatment with by intragastric gavage. Pilocarpine-induced saliva was collected to determine salivary flow rate, pH, buffering capacity, and biochemical composition. Analyses of histomorphometric parameters and redox balance markers were performed on the parotid and submandibular glands.
Salivary flow rate, pH, buffering capacity, total protein, potassium, sodium, and chloride were similar between groups. However, phosphate and calcium were reduced in VPA400, while amylase was increased in both VPA100 and VPA400. We did not detect significant differences in the areas of acini, ducts, and connective tissue in the salivary glands between the groups. There were no significant changes in the redox status of the submandibular glands. In turn, in the parotid glands we detected reduced total oxidizing capacity and lipid peroxidation, measured as thiobarbituric acid reactive substances (TBARs) and higher uric acid concentration in both the VPA100 and VPA400 groups, and increased superoxide dismutase (SOD) in the VPA400 group.
Chronic treatment with VPA modified the salivary biochemical composition and caused disruption in the redox state of the parotid gland in rats.
使用生化、功能、组织形态计量学和氧化还原状态参数，研究抗惊厥药物丙戊酸（VPA）对雄性大鼠唾液腺的影响。
将24只雄性Wistar大鼠随机分为三组（每组n = 8）：对照组（0.9%盐溶液）、VPA100组（100 mg/kg）和VPA400组（400 mg/kg）。连续21天通过灌胃给药进行治疗。收集毛果芸香碱诱导的唾液，以测定唾液流速、pH值、缓冲能力和生化成分。对腮腺和颌下腺进行组织形态计量学参数和氧化还原平衡标志物分析。
各组之间的唾液流速、pH值、缓冲能力、总蛋白、钾、钠和氯相似。然而，VPA400组的磷酸盐和钙减少，而VPA100组和VPA400组的淀粉酶均增加。我们未检测到各组唾液腺腺泡、导管和结缔组织面积的显著差异。颌下腺的氧化还原状态没有显著变化。相反，在腮腺中，我们检测到VPA100组和VPA400组的总氧化能力降低和脂质过氧化（以硫代巴比妥酸反应性物质（TBARs）衡量），尿酸浓度升高，VPA400组的超氧化物歧化酶（SOD）增加。
VPA的慢性治疗改变了大鼠唾液的生化成分，并导致腮腺氧化还原状态的破坏。
