Rosenthal S N, Bardfeld P A
Nucl Med Commun. 1986 Jul;7(7):511-7. doi: 10.1097/00006231-198607000-00006.
Technetium-99m-labelled colloids covering the entire spectrum of particle size were prepared from commercial kits (antimony colloid, sulphur colloid, tin colloid) or by chemical (excess aluminium) or thermal modification of sulphur colloid (heating time of 30 min). Particle size measurements were obtained using electron microscopy and/or a laser light scattering technique. The results for antimony colloid agreed reasonably well for both techniques with a range of 3 to 25 nm. However, standard sulphur colloid had a smaller size (220 nm) when measured by electron microscopy in comparison to the larger size (390 nm) obtained with the light scattering method. This discrepancy was felt to be due to artefacts created by electron microscopy. These artefacts (drying and sublimation) were avoided by the laser light scattering techniques which has the advantage of being non-destructive and of quickly sizing particles while still in solution. The larger colloidal particles were sulphur colloid doped with aluminium ions (1000 nm) and the tin colloid (2500 nm) which were sized by electron microscopy and laser light scattering respectively.
从商业试剂盒（锑胶体、硫胶体、锡胶体）或通过对硫胶体进行化学（过量铝）或热改性（加热时间30分钟）制备了涵盖整个粒径范围的锝-99m标记胶体。使用电子显微镜和/或激光散射技术进行粒径测量。两种技术测得的锑胶体结果相当吻合，粒径范围为3至25纳米。然而，标准硫胶体通过电子显微镜测量时尺寸较小（220纳米），而通过光散射法测得的尺寸较大（390纳米）。这种差异被认为是由电子显微镜产生的假象所致。激光散射技术避免了这些假象（干燥和升华），其优点是无损，并且能在颗粒仍处于溶液状态时快速测定其尺寸。较大的胶体颗粒是分别通过电子显微镜和激光散射法测定尺寸的掺杂铝离子的硫胶体（1000纳米）和锡胶体（2500纳米）。
